PMID: 6407008Jun 1, 1983Paper

Cleavage of chromatin with methidiumpropyl-EDTA . iron(II)

Proceedings of the National Academy of Sciences of the United States of America
I L CartwrightS C Elgin

Abstract

Methidiumpropyl-EDTA . iron(II) [MPE . Fe (II)] cleaves double-helical DNA with considerably lower sequence specificity than micrococcal nuclease. Moreover, digestions with MPE . Fe(II) can be performed in the presence of certain metal chelators, which will minimize the action of many endogenous nucleases. Because of these properties MPE . Fe(II) would appear to be a superior tool for probing chromatin structure. We have compared the patterns generated from the 1.688 g/cm3 complex satellite, 5S ribosomal RNA, and histone gene sequences of Drosophila melanogaster chromatin and protein-free DNA by MPE . Fe(II) and micrococcal nuclease cleavage. MPE . Fe(II) at low concentrations recognizes the nucleosome array, efficiently introducing a regular series of single-stranded (and some double-stranded) cleavages in chromatin DNA. Subsequent S1 nuclease digestion of the purified DNA produces a typical extended oligonucleosome pattern, with a repeating unit of ca. 190 base pairs. Under suitable conditions, relatively little other nicking is observed. Unlike micrococcal nuclease, which has a noticeable sequence preference in introducing cleavages, MPE . Fe(II) cleaves protein-free tandemly repetitive satellite and 5S DNA sequences in a ne...Continue Reading

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·R P LiftonD S Hogness
Jan 12, 1978·Nature·G Felsenfeld
Dec 1, 1977·Cell·S Artavanis-TsakonasW J Gehring
Dec 1, 1981·Cell·P N BryanM L Birnstiel
Jun 1, 1981·Cell·H G Zachau, T Igo-Kemenes
Jan 29, 1980·Biochemical and Biophysical Research Communications·S A Nedospasov, G P Georgiev
Jun 25, 1981·Nucleic Acids Research·C DingwallR A Laskey
Jan 1, 1982·Annual Review of Biochemistry·T Igo-KemenesH G Zachau
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M W Van DykeP B Dervan
Jan 1, 1982·CRC Critical Reviews in Biochemistry·I L CartwrightG C Howard
Jan 1, 1980·Annual Review of Biochemistry·J D McGhee, G Felsenfeld
May 1, 1980·Quarterly Reviews of Biophysics·A D Mirzabekov
Jun 25, 1981·Nucleic Acids Research·W Hörz, W Altenburger

❮ Previous
Next ❯

Citations

Jan 4, 2003·Chembiochem : a European Journal of Chemical Biology·Carolina CarrascoChristian Bailly
Aug 31, 2001·Journal of Cellular Biochemistry·Y Chen, J M Keller
Feb 1, 1990·Journal of Molecular Recognition : JMR·P E Nielsen
Sep 1, 1983·Developmental Biology·K LowenhauptS C Elgin
Feb 28, 1992·Biochimica Et Biophysica Acta·F Thoma
Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·M TrussM Beato
Jan 1, 1992·Trends in Biochemical Sciences·J H WhiteW R Bauer
Mar 17, 2004·Biochimica Et Biophysica Acta·H Karimi Kinyamu, Trevor K Archer
Mar 7, 2002·Biochimie·V MoralesH Richard-Foy
Apr 1, 1996·Current Opinion in Genetics & Development·S C Elgin
May 5, 2001·The Biochemical Journal·V K RakyanE Whitelaw
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M J Solomon, A Varshavsky
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C H Chen, D S Sigman
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C T McMurray, K E van Holde
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·J BartschM Beato
Jan 11, 1985·Nucleic Acids Research·L Kunnath, J Locker
Jun 26, 1989·Nucleic Acids Research·A E RobersonD E Olins
Jul 11, 1992·Nucleic Acids Research·H U EnrightR P Hebbel
Dec 24, 2010·Genome Research·Sevinç ErcanJason D Lieb
May 15, 1987·European Journal of Biochemistry·R L AdamsR Eason
Jun 1, 1988·European Journal of Biochemistry·M Pagès, G Roizès
Jul 1, 1992·Trends in Biochemical Sciences·J J Hayes, A P Wolffe
Jun 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Haruhiko IshiiBing Ren
Mar 22, 2014·FEBS Open Bio·Amrita BanerjeeDipak Dasgupta
Mar 8, 2005·Experimental Cell Research·Per-Henrik HolmqvistOrjan Wrange
May 1, 1984·Cell·M Ryoji, A Worcel
Apr 19, 2007·Annals of Medicine·Zachary KaminskyArturas Petronis
Mar 1, 1995·Chemistry & Biology·J J Hayes
May 1, 1997·Methods : a Companion to Methods in Enzymology·J S MymrykT K Archer
Jan 1, 1986·CRC Critical Reviews in Biochemistry·R S WuW M Bonner
Jan 1, 1984·CRC Critical Reviews in Biochemistry·S Neidle, Z Abraham
Nov 25, 1984·Journal of Molecular Biology·C A Edwards, R A Firtel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.