Cleavage of E-Cadherin by Matrix Metalloproteinase-7 Promotes Cellular Proliferation in Nontransformed Cell Lines via Activation of RhoA.

Journal of Oncology
Conor C LynchBarbara Fingleton

Abstract

Perturbations in cell-cell contact machinery occur frequently in epithelial cancers and result in increased cancer cell migration and invasion. Previously, we demonstrated that MMP-7, a protease implicated in mammary and intestinal tumor growth, can process the adherens junction component E-cadherin. This observation leads us to test whether MMP-7 processing of E-cadherin could directly impact cell proliferation in nontransformed epithelial cell lines (MDCK and C57MG). Our goal was to investigate the possibility that MMP-7 produced by cancer cells may have effects on adjacent normal epithelium. Here, we show that MMP-7 processing of E-cadherin mediates, (1) loss of cell-cell contact, (2) increased cell migration, (3) a loss of epithelial cell polarization and (4) increased cell proliferation via RhoA activation. These data demonstrate that MMP-7 promotes epithelial cell proliferation via the processing of E-cadherin and provide insights into the molecular mechanisms that govern epithelial cell growth.

References

Aug 1, 1990·Environmental Health Perspectives·R J WieserF Oesch
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C L WilsonL M Matrisian
May 16, 1998·The Journal of Biological Chemistry·R B Hazan, L Norton
Apr 16, 1998·Nature·P GuilfordA E Reeve
Mar 15, 2001·Cancer Letters·S Aznar, J C Lacal
Jul 18, 2001·The Journal of Biological Chemistry·N K NorenK Burridge
Aug 4, 2001·Cancer Letters·K Pruitt, C J Der
Sep 7, 2001·Current Opinion in Cell Biology·P Z Anastasiadis, A B Reynolds
Dec 6, 2001·Nature Reviews. Molecular Cell Biology·M Fukata, K Kaibuchi
Jan 5, 2002·Neoplasia : an International Journal for Oncology Research·B FingletonL M Matrisian
Apr 4, 2002·Biological Chemistry·Filip RyniersErik Bruyneel
Dec 21, 2002·Differentiation; Research in Biological Diversity·Conor C Lynch, Lynn M Matrisian
May 20, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L Di Croce, P G Pelicci
Oct 2, 2003·Current Opinion in Cell Biology·Margaret J Wheelock, Keith R Johnson
Feb 18, 2004·Nature Reviews. Cancer·Ugo Cavallaro, Gerhard Christofori
Dec 14, 2004·Biochimica Et Biophysica Acta·Salvador A BenitahJuan Carlos Lacal
Jan 18, 2005·Experimental Cell Research·Permila C HarrellLynn M Matrisian
May 17, 2005·Cancer Cell·Conor C LynchMitsuru Futakuchi
Jun 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Thorsten MaretzkyPaul Saftig
Feb 17, 2006·Genes & Development·Vasilena GochevaJohanna A Joyce
Feb 23, 2007·Current Pharmaceutical Design·Barbara Fingleton
May 18, 2007·Nature Reviews. Cancer·Héctor PeinadoAmparo Cano
Jun 26, 2007·Cells, Tissues, Organs·Joanne Masterson, Shirley O'Dea
Oct 24, 2007·Clinical & Experimental Metastasis·Olivier De WeverMarc Bracke
Mar 22, 2008·The Journal of Biological Chemistry·Emma C FerberYasuyuki Fujita
Mar 28, 2008·Biochemical Society Transactions·W James Nelson
Apr 25, 2008·The Journal of Biological Chemistry·Abdo J NajyMark L Day
Jul 2, 2008·Expert Reviews in Molecular Medicine·Abde M Abukhdeir, Ben Ho Park
Sep 16, 2008·The Journal of Pharmacology and Experimental Therapeutics·Fernanda LeveJosé Andrés Morgado-Díaz
Nov 19, 2008·The Journal of Cell Biology·Edwin SotoPanos Z Anastasiadis
Dec 17, 2009·Nature Cell Biology·Elena KardashErez Raz
Feb 6, 2010·Methods in Molecular Biology·Sean E GillWilliam C Parks

❮ Previous
Next ❯

Citations

Oct 12, 2012·Apoptosis : an International Journal on Programmed Cell Death·Kinga Musiał, Danuta Zwolińska
Apr 27, 2012·International Journal of Cell Biology·Jimmie E FataMarcia V Fournier
Feb 22, 2012·Biochimica Et Biophysica Acta·Massimiliano CuccioloniMauro Angeletti
Sep 27, 2013·Journal of Dental Research·X Guan, J D Bartlett
Oct 12, 2012·Journal of Dental Research·J D Bartlett, C E Smith
Aug 1, 2015·Molecular Biology of the Cell·Ryuta KamekuraAsma Nusrat
Feb 9, 2012·Onkologie·Marek PytliakViola Mechírová
Oct 25, 2017·Journal of Cell Science·Jesús Gómez-EscuderoAlicia G Arroyo
Mar 21, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kasturi ChatterjeeSnehasikta Swarnakar
Apr 29, 2011·Cells, Tissues, Organs·John D BartlettCharles E Smith
Aug 6, 2020·International Journal of Molecular Sciences·Vidhi MalikRenu Wadhwa
Apr 26, 2020·Cells·Michele Sommariva, Nicoletta Gagliano
Sep 20, 2011·Journal of Agricultural and Food Chemistry·Meng-Chiu LinFeng-Yao Tang

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
pull-down
immunodepletion

Software Mentioned

Prism

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.