PMID: 6170987Sep 1, 1981Paper

Cleavage of four avian sarcoma virus polyproteins with virion protease p15 removes gag sequences and yields large fragments that function as tyrosine phosphoacceptors in vitro

Proceedings of the National Academy of Sciences of the United States of America
J GhysdaelP K Vogt

Abstract

The transformation-specific polyproteins of avian sarcoma viruses PRCII, PRCII-p, Fujinami sarcoma virus (FSV), and Esh sarcoma virus (ESV) consist of two domains, one derived from a partial viral gag gene and the other representing an apparently cell-derived insert in the defective viral genome. These gag-linked proteins were cleaved with retrovirion protease p15. Cleavage of PRCII-p polyprotein P170, P105 of PRCII, and P140 of FSV occurred within the gag domain and generated fragments of Mr 130,000, 70,000, and 115,000, respectively, containing all of the transformation-specific sequences linked to a remnant of the original gag sequences. ESV P80 was cleaved inside the transformation-specific domain, yielding a Mr 35,000--38,000 fragment from the NH2-terminal half of the molecule consisting of the entire gag portion and some no-gag sequences and a Mr 48,000 fragment containing most of the transformation-specific sequences. The tyrosine phosphorylation sites of the polyproteins were found in every case in the transformation-specific fragments. The major serine phosphorylation site of ESV P80 was found to reside in the Mr 35,000--38,000 gag-containing fragment, probably within the transformation-specific sequences of that cleav...Continue Reading

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K von der Helm
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M S Collett, R L Erikson
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W H LeeP H Duesberg
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T HanafusaH Hanafusa
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S KawaiK Toyoshima
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M ShibuyaJ R Stephenson
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J C NeilP K Vogt
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J GhysdaelP K Vogt

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Citations

Apr 1, 2011·Journal of Biosciences·Jana Rohozková, Milan Navrátil
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B G NeelW S Hayward

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