Cleavage of GPI-anchored proteins from the plasma membrane activates apical endocytosis in pancreatic acinar cells

European Journal of Cell Biology
S D FreedmanG A Scheele

Abstract

Using rat pancreatic acini, we have recently shown that apical endocytosis is inhibited at pH 6.0 and progressively activated as the pH is increased to 8.3. Endocytotic activity correlated with the release of GP2, a GPI-linked protein, from the apical plasma membrane. To determine whether the cleavage of GPI-anchored proteins from the membrane of rat acinar cells was responsible for activation of endocytosis, cells at pH 6.0 were incubated with PI-specific phospholipase C (PI-PLC). PI-PLC treatment reversed the inhibition of endocytosis observed at pH 6.0. Reactivation of endocytosis correlated with PI-PLC-induced release of GP2 but not cleavage of phospholipids in cellular membranes. Furthermore, administration of diacylglycerol or phorbol esters had no effect on reactivation of endocytosis. PI-PLC did not alter intracellular pH or calcium levels. Two proteins were identified as GPI-linked proteins on the cell surface. One was GP2, whose release from the apical plasma membrane correlated with apical endocytosis of horseradish peroxidase (HRP). The other protein, identified by Western blotting using an antibody directed against a cryptic determinant exposed on GPI-linked proteins after cleavage with PI-PLC, has a molecular weig...Continue Reading

Citations

Jul 30, 2011·Journal of Biosciences·Madhusudhan BudathaAparna Dutta-Gupta
Apr 19, 2002·Pancreas·Benjamin A FritzAnson W Lowe
Nov 27, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philippe NourissatOdile Sergent
Jun 25, 2015·Histochemistry and Cell Biology·Miguel ArosoMichael Schrader
Aug 18, 2004·Biochemical and Biophysical Research Communications·Su YuAnson W Lowe
Jan 27, 2017·Annual Review of Immunology·Yosuke Kurashima, Hiroshi Kiyono
Feb 24, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Diana D H ThomasGuy E Groblewski
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Frank Thévenod
Feb 10, 1999·The American Journal of Physiology·S D FreedmanA Gelrud
Dec 31, 2002·Biochemical and Biophysical Research Communications·Frank ThévenodCatherine M Fuller
Jun 29, 2000·The Medical Clinics of North America·S D FreedmanJ G Alvarez
Sep 25, 1998·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·S D Freedman

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