PMID: 9525682Apr 3, 1998Paper

Cleavage of human immunodeficiency virus type 1 proteinase from the N-terminally adjacent p6* protein is essential for efficient Gag polyprotein processing and viral infectivity

Journal of Virology
U Tessmer, H G Kräusslich

Abstract

Maturation of infectious human immunodeficiency virus (HIV) particles requires proteolytic cleavage of the structural polyproteins by the viral proteinase (PR), which is itself encoded as part of the Gag-Pol polyprotein. Expression of truncated PR-containing sequences in heterologous systems has mostly led to the autocatalytic release of an 11-kDa species of PR which is capable of processing all known cleavage sites on the viral precursor proteins. Relatively little is known about cleavages within the nascent virus particle, on the other hand, and controversial results concerning the active PR species inside the virion and the relative activities of extended PR species have been reported. Here, we report that HIV type 1 (HIV-1) particles of four different strains obtained from different cell lines contain an 11-kDa PR, with no extended PR proteins detectable. Furthermore, mutation of the N-terminal PR cleavage site leading to production of an N-terminally extended 17-kDa PR species caused a severe defect in Gag polyprotein processing and a complete loss of viral infectivity. We conclude that N-terminal release of PR from the HIV-1 polyprotein is essential for viral replication and suggest that extended versions of PR may have a...Continue Reading

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Citations

Apr 18, 2009·Current Opinion in HIV and AIDS·Monique NijhuisCharles Ab Boucher
Aug 3, 1999·European Journal of Biochemistry·B MahalingamI T Weber
Oct 10, 2002·FEBS Letters·Petra Schlick, Tim Skern
Oct 31, 2003·AIDS Research and Human Retroviruses·N WhitehurstA Gamarnik
Feb 9, 2010·The Biochemical Journal·Maximilian J HartlBirgitta M Wöhrl
Jul 19, 2013·Future Medicinal Chemistry·Liangqun Huang, Chaoping Chen
Oct 18, 2005·AIDS Research and Human Retroviruses·Africa HolguínVincent Soriano
Sep 24, 1999·Journal of Biomedical Science·Y Y ChangW J Syu
Aug 7, 1999·The Journal of Biological Chemistry·J M LouisN T Nashed
Jul 12, 2018·Scientific Reports·Jana HumpolíčkováTaťána Majerová
May 16, 2002·Proteins·Bhuvaneshwari MahalingamIrene T Weber

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