Cleavage of osteopontin by matrix metalloproteinase-12 modulates experimental autoimmune encephalomyelitis disease in C57BL/6 mice.

The American Journal of Pathology
Angelika Goncalves DaSilvaV Wee Yong

Abstract

A role for osteopontin (OPN) in promoting disease activity of multiple sclerosis or its animal model experimental autoimmune encephalomyelitis (EAE) has recently been suggested. As the biological activity of OPN is heavily influenced by posttranslational processing, we investigated the capacity of matrix metalloproteinase (MMP)-12 to cleave OPN and determined whether this influenced disease activity. We found that OPN mRNA and protein expression in the spinal cord increased with EAE disease in C57BL/6 mice concurrently with MMP-12 expression. A Western blot of EAE and control spinal cords revealed different OPN-immunoreactive bands, with a pattern that was similar to MMP-12 cleavage of recombinant OPN in vitro. In addition, OPN fragments in the spinal cord of EAE-afflicted mice were reduced in MMP-12(-/-) mice compared with wild-type controls. However, examination of OPN(-/-) mice in short- and long-term experiments revealed no difference in EAE outcomes from wild-type animals. OPN/MMP-12 double null mice were generated, and it was revealed that MMP-12(-/-) mice had a worsening of disease compared with wild-type mice, which returned to wild-type levels in the OPN/MMP-12 double null mice. These results suggest that EAE disease a...Continue Reading

References

Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·J M ShipleyS D Shapiro
Jan 28, 1998·Acta Neuropathologica·J A CossinsC J De Groot
Apr 29, 1998·The Journal of Clinical Investigation·L LiawB L Hogan
Oct 6, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J SodekM D McKee
Jul 4, 2001·Nature Reviews. Neuroscience·V W YongD R Edwards
Apr 17, 2002·Lancet·Alastair Compston, Alasdair Coles
Dec 7, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Gary A Rosenberg
Mar 7, 2003·Journal of Neuroimmunology·Masaaki NiinoKunio Tashiro
May 23, 2003·Genes and Immunity·S CaillierUNKNOWN Multiple Sclerosis Genetics Group
Jun 5, 2003·Annals of Neurology·Mario H J VogtLex Nagelkerken
Jul 5, 2003·The Journal of Surgical Research·Hideki MasudaTadatoshi Takayama
Jul 23, 2003·American Journal of Physical Medicine & Rehabilitation·Michael D KaufmanH James Norton
Sep 27, 2003·Biochemical and Biophysical Research Communications·Yoshinosuke HamadaNariaki Matsuura
Jan 7, 2004·Biochemical and Biophysical Research Communications·Satoshi MochidaKenji Fujiwara
Apr 15, 2004·Molecular and Cellular Neurosciences·Raghuram SelvarajuUrsula Boschert
Dec 14, 2004·Journal of Neuroimmunology·Fabrizio GiulianiV Wee Yong
Aug 6, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew WeaverV Wee Yong
Mar 3, 2006·The New England Journal of Medicine·Elliot M FrohmanCedric S Raine
Mar 21, 2006·Acta Neuropathologica·Maria Diaz-SanchezMargaret M Esiri
Dec 21, 2006·Nature Immunology·Ingunn M Stromnes, Joan M Goverman
Mar 27, 2007·Journal of the Neurological Sciences·V Wee YongLuanne M Metz
Jul 25, 2007·Seminars in Cell & Developmental Biology·Smriti M AgrawalV Wee Yong
Sep 21, 2007·Annals of Neurology·Nathalie KoningInge Huitinga
May 14, 2008·Archives of Neurology·Manjit BraitchCris S Constantinescu

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Citations

Sep 5, 2013·Acta neurologica Belgica·Mohammad A JavaidZeeshan Sheikh
Apr 29, 2011·European Heart Journal·Stelios PsarrasYassemi Capetanaki
Sep 6, 2011·Progress in Neurobiology·Minesh Kapadia, Boris Sakic
Mar 11, 2016·The American Journal of Pathology·Mutsuki KuraokaShin'ichi Takeda
Jul 16, 2015·Canadian Journal of Physiology and Pharmacology·Merry L LindseyLisandra E de Castro Brás
Apr 19, 2015·Critical Care : the Official Journal of the Critical Care Forum·Yohei HiranoPing Wang
Jun 22, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ralf G RempeBjörn Bauer
Aug 7, 2019·Cells·Anne-Sophie LamortGeorgios T Stathopoulos
Jun 6, 2019·Cellular and Molecular Life Sciences : CMLS·Sameeksha ChopraAntoine Dufour
Jan 18, 2017·Journal of Immunology Research·Nausicaa ClementeAnnalisa Chiocchetti
Dec 4, 2020·Neural Regeneration Research·Giuseppe CappellanoCristoforo Comi
May 28, 2020·Journal of Medicinal Chemistry·Pauline ZipfelPatrick Dallemagne

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