Cleavage of peptide hormones by alpha 2-macroglobulin-trypsin complex and its relation to the pathogenesis and chemotherapy of acute pancreatitis

Clinica Chimica Acta; International Journal of Clinical Chemistry
J Hermon-TaylorJ Dunham

Abstract

Access to the active site of alpha 2-macroglobulin bound proteinases by macromolecular substrates and inhibitors is likely to be influenced by steric favourability and flexibility, as well as by molecular size. Hydrolysis of pure porcine cholecystokinin-pancreozymin 39, a flexible single chain peptide, by alpha 2-macroglobulin-trypsin complex resulted in a rapid up to 6-fold increase in cholecystokinin bioactivity; alpha 2-macroglobulin-trypsin rapidly abolished the bioactivity of endogenous parathormone in human plasma. Inhibition of both reactions was completed by low concentrations of antipain and leupeptin; Trasylol (aprotinin), a single chain peptide with three disulphide bonds, was an ineffective inhibitor even in massive molar excess. These findings may provide an explanation for the disordered calcium homeostasis in severe acute pancreatitis and for the failure of Trasylol to reduce mortality; they suggest that sterically favourable low molecular weight inhibitors may provide effective specific chemotherapy for the disease.

References

Mar 11, 1977·Virchows Archiv. A, Pathological Anatomy and Histology·M Lampel, H F Kern
Mar 4, 1976·The New England Journal of Medicine·G M RobertsonH L Estep
Feb 1, 1978·The American Journal of Digestive Diseases·M C GeokasC Largman
Oct 1, 1978·The British Journal of Surgery·C W ImrieA J McKay
Oct 1, 1978·The Journal of Endocrinology·C E MarshallA G Johnson
Oct 1, 1978·Clinical Endocrinology·D J ChambersJ Chayen
Mar 1, 1975·The British Journal of Surgery·G Glazer
Feb 1, 1975·The British Journal of Surgery·J R CondonJ Day

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Citations

Jun 16, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Sølling
Oct 1, 1987·The British Journal of Surgery·T LeeseP R Bell
Jan 1, 1987·American Journal of Surgery·I W BrowderN DiLuzio
Mar 1, 1994·The British Journal of Surgery·C McKayJ N Baxter
Jan 1, 1985·Scandinavian Journal of Gastroenterology. Supplement·J Hermon-Taylor, G C Heywood
Mar 1, 1987·The British Journal of Surgery·P K Donnelly
Oct 1, 1983·The British Journal of Surgery·P K DonnellyR M Taylor
Sep 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A LassonK Ohlsson
Aug 1, 1986·The British Journal of Surgery·T R Terry
Oct 1, 1981·International Journal of Peptide and Protein Research·L Karic, C B Glaser
Oct 1, 1986·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·D Grant

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