Cleavage of RNA hairpins mediated by a developmentally regulated CCCH zinc finger protein.

Molecular and Cellular Biology
C Bai, P P Tolias

Abstract

Control of RNA turnover is a major, but poorly understood, aspect of gene regulation. In multicellular organisms, progress toward dissecting RNA turnover pathways has been made by defining some cis-acting sequences that function as either regulatory or cleavage targets (J. G. Belasco and G. Brawerman, Control of Messenger RNA Stability, 1993). However, the identification of genes encoding proteins that regulate or cleave target RNAs has been elusive (C. A. Beelman and R. Parker, Cell 81:79-183, 1995); this gap in knowledge has made it difficult to identify additional components of RNA turnover pathways. We have utilized a modified expression cloning strategy to identify a developmentally regulated gene from Drosophila melanogaster that encodes a RNase that we refer to as Clipper (CLP). Significant sequence matches to open reading frames encoding unknown functions identified from the Caenorhabditis elegans and Saccharomyces cerevisiae genome sequencing projects suggest that all three proteins are members of a new protein family conserved from lower eukaryotes to invertebrates. We demonstrate that a member of this new protein family specifically cleaves RNA hairpins and that this activity resides in a region containing five copie...Continue Reading

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Citations

Apr 15, 2009·The Journal of Cell Biology·Jessica A HurtPamela A Silver
Jun 11, 2015·Biomolecules·Manohar Chakrabarti, Arthur G Hunt
Sep 22, 2011·Wiley Interdisciplinary Reviews. RNA·Zbigniew Dominski
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Apr 30, 2021·ELife·Chris EstellSteven West
Nov 20, 2004·Science·Marvin Wickens, Tania N Gonzalez
Aug 17, 2006·Genes & Development·Ekaterini A KritikouMichael O Hengartner

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