PMID: 8948651Nov 15, 1996Paper

Cleavage of single- and double-stranded DNAs containing an abasic residue by Escherichia coli exonuclease III (AP endonuclease VI)

Nucleic Acids Research
T ShidaJ Sekiguchi

Abstract

The Escherichia coli exonuclease III (AP endonuclease VI) is a DNA-repair enzyme that hydrolyzes the phosphodiester bond 5' to an abasic site in DNA. To study how the enzyme recognizes the abasic site, we used oligonucleotides containing a synthetic abasic site at any desired position in the sequence. We prepared oligonucleotides containing an abasic residue such as 2'-deoxyribosylformamide, 2'-deoxyribose, 1',2'-dideoxy ribofuranose or propanediol. Duplex oligonucleotides containing an abasic residue used in this study were cleaved on the 5' side of the abasic site by exonuclease III in spite of the varieties of the bases opposite and adjacent to the abasic site. In addition, we observed that the enzyme cleaved single-stranded oligonucleotides containing an abasic site on the 5' side of the abasic site. These findings suggest that the enzyme may principally recognize the DNA-pocket formed at an abasic site. The indole ring of the tryptophan 212 residue of the exonuclease III is probably intercalated to the abasic site. The tryptophan in the vicinity of the catalytic site is conserved in the type II AP endonuclease from various organisms.

References

Sep 1, 1990·Mutation Research·P W Doetsch, R P Cunningham
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M SanderA Rich
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B DempleD S Chen
Sep 1, 1990·Mutation Research·K Sakumi, M Sekiguchi
Jan 1, 1988·Environmental and Molecular Mutagenesis·S S Wallace
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y W Kow, S S Wallace
Jan 1, 1985·Progress in Nucleic Acid Research and Molecular Biology·F Hutchinson
Dec 15, 1987·Biochemistry·Y W Kow, S S Wallace
Jan 1, 1986·Annual Review of Genetics·L A Loeb, B D Preston
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H R WarnerS Linn
Jan 1, 1982·Annual Review of Biochemistry·T Lindahl
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T BehmoarasC Helene
Jan 1, 1994·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·N OgasawaraH Yoshikawa
Jul 7, 1995·The Journal of Biological Chemistry·D M WilsonB Demple

❮ Previous
Next ❯

Citations

Nov 24, 1999·Journal of Biomolecular Structure & Dynamics·L AyadiR Lavery
Mar 4, 2000·Journal of Biomolecular Structure & Dynamics·L AyadiR Lavery
Aug 19, 2007·Nucleic Acids Research·Gemma Serrano-HerasMargarita Salas
May 4, 2010·Journal of Molecular Biology·Kristina LakomekRalf Ficner
Nov 1, 2005·Biochimica Et Biophysica Acta·Xipeng Liu, Jianhua Liu
Nov 11, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Huaijun NieTao Yang
Dec 17, 2002·Oncogene·Laurent GrosJacques Laval
Jan 20, 2006·The Journal of Biological Chemistry·Gemma Serrano-HerasAlicia Bravo
Dec 28, 2018·International Journal of Molecular Sciences·Wei-Wei WangXi-Peng Liu
Sep 28, 2001·Nucleic Acids Research·N E BroudeD Englert
Feb 1, 2013·EcoSal Plus·Sophie CouvéMurat Saparbaev
Jul 22, 2021·Computational and Structural Biotechnology Journal·Tung-Chang LiuYu-Yuan Hsiao
Feb 19, 2002·Chemical Reviews·Sheila S. David, Scott D. Williams

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.