PMID: 9525628Apr 3, 1998Paper

Cleavage of the feline calicivirus capsid precursor is mediated by a virus-encoded proteinase

Journal of Virology
Stanislav V SosnovtsevK Y Green

Abstract

Feline calicivirus (FCV), a member of the Caliciviridae, produces its major structural protein as a precursor polyprotein from a subgenomic-sized mRNA. In this study, we show that the proteinase responsible for processing this precursor into the mature capsid protein is encoded by the viral genome at the 3'-terminal portion of open reading frame 1 (ORF1). Protein expression studies of either the entire or partial ORF1 indicate that the proteinase is active when expressed either in in vitro translation or in bacterial cells. Site-directed mutagenesis was used to characterize the proteinase Glu-Ala cleavage site in the capsid precursor, utilizing an in vitro cleavage assay in which mutant precursor proteins translated from cDNA clones were used as substrates for trans cleavage by the proteinase. In general, amino acid substitutions in the P1 position (Glu) of the cleavage site were less well tolerated by the proteinase than those in the P1' position (Ala). The precursor cleavage site mutations were introduced into an infectious cDNA clone of the FCV genome, and transfection of RNA derived from these clones into feline kidney cells showed that efficient cleavage of the capsid precursor by the virus-encoded proteinase is a critical...Continue Reading

References

May 1, 1977·Journal of Virology·D W Ehresmann, F L Schaffer
Aug 10, 1978·Nature·D N BlackF Brown
Sep 1, 1992·Biochemistry·D A JewellB A Malcolm
May 1, 1991·Archives of Biochemistry and Biophysics·J R Weidner, B M Dunn
Oct 1, 1991·Journal of Virology·J D NeillR L Heinrikson
Jan 1, 1990·Archives of Virology·M J Carter
Jan 1, 1990·Annual Review of Microbiology·A C Palmenberg
Jul 1, 1993·Virology·X JiangM K Estes
Feb 1, 1996·Journal of Virology·J M Martín AlonsoF Parra

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Citations

May 11, 2000·The Journal of Infectious Diseases·I N Clarke, P R Lambden
Jun 20, 2001·The Journal of General Virology·Y MatsuuraT Sugimura
Apr 14, 2004·Journal of Virology·Tanaji MitraKim Y Green
May 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kyeong-Ok ChangKim Y Green
Feb 14, 2007·Veterinary Research·Alan D RadfordRosalind M Gaskell
May 3, 2020·Transboundary and Emerging Diseases·Barbara Di MartinoVito Martella
Mar 16, 2005·Journal of Virology·Stanislav V SosnovtsevKim Y Green
Nov 30, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mohamed Abd-EldaimMelissa Kennedy
May 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rong ChenB V Venkataram Prasad
May 16, 2015·Archives of Virology·Fa-Ming MiaoRong-Liang Hu
May 15, 2020·Journal of Virology·Christian Urban, Christine Luttermann
Jan 21, 2000·The Journal of General Virology·Y MatsuuraT Sugimura
Sep 19, 2003·The Journal of General Virology·Angelika OehmigEberhard Pfaff
Aug 7, 2002·Journal of Virology·Kim Y GreenStanislav V Sosnovtsev
Jul 18, 2008·Journal of Virology·Kyeong-Ok ChangStanislav V Sosnovtsev
Jul 16, 2004·Journal of Virology·Muge Kuyumcu-MartinezRichard E Lloyd
Mar 26, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·K GeisslerU Truyen
Dec 16, 2006·Virus Genes·Charlie C HsuRobert S Livingston
Dec 28, 2012·Journal of Virology·Eugenio J AbenteKim Y Green
Apr 15, 2003·The Journal of General Virology·Naema Al-MolawiLisa O Roberts
May 28, 2004·Journal of Virology·Rong ChenB V Venkataram Prasad
Oct 13, 2006·The Journal of General Virology·Tomoichiro OkaNaokazu Takeda
Oct 22, 2019·Frontiers in Immunology·Yoatzin Peñaflor-TéllezAna Lorena Gutiérrez-Escolano

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