Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - β1 (TGF-β1) and potential effects on migration and invasion

BMC Cancer
Synnøve MagnussenGunbjørg Svineng

Abstract

Urokinase plasminogen activator (uPA) receptor (uPAR) is up-regulated at the invasive tumour front of human oral squamous cell carcinoma (OSCC), indicating a role for uPAR in tumour progression. We previously observed elevated expression of uPAR at the tumour-stroma interface in a mouse model for OSCC, which was associated with increased proteolytic activity. The tumour microenvironment regulated uPAR expression, as well as its glycosylation and cleavage. Both full-length- and cleaved uPAR (uPAR (II-III)) are involved in highly regulated processes such as cell signalling, proliferation, migration, stem cell mobilization and invasion. The aim of the current study was to analyse tumour associated factors and their effect on uPAR cleavage, and the potential implications for cell proliferation, migration and invasion. Mouse uPAR was stably overexpressed in the mouse OSCC cell line AT84. The ratio of full-length versus cleaved uPAR as analysed by Western blotting and its regulation was assessed by addition of different protease inhibitors and transforming growth factor - β1 (TGF-β1). The role of uPAR cleavage in cell proliferation and migration was analysed using real-time cell analysis and invasion was assessed using the myoma inva...Continue Reading

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Citations

Jul 24, 2020·Scientific Reports·Synnøve Norvoll MagnussenGunbjørg Svineng
Jan 12, 2019·Biomedit︠s︡inskai︠a︡ khimii︠a︡·E V KugaevskayaN I Solovyeva
Apr 27, 2021·Cancer Biomarkers : Section a of Disease Markers·Yingzhu KangPing Zhang
Jun 1, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sijing LiLi Sun

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Methods Mentioned

BETA
electrophoresis
transfection
Fluorescence-activated cell sorting
scraping
glycosylation
PCR
reverse
flow cytometry

Software Mentioned

xCELLigence
FlowJo
SPSS Statistics
Leica Application Suite (
Systat
Leica Application Suite
SigmaPlot
SPSS
IM50
Olympus DP

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