Cleaved SLPI, a novel biomarker of chymase activity

Biological Chemistry
S M BelkowskiM R D'Andrea

Abstract

Secretory leukocyte protease inhibitor (SLPI) is a protease inhibitor of the whey acidic protein-like family inhibiting chymase, chymotrypsin, elastase, proteinase 3, cathepsin G and tryptase. Performing in vitro enzymatic assays using both Western blotting and liquid chromatography/mass spectrometry techniques we showed that, of the proteases known to interact with SLPI, only chymase could uniquely cleave this protein. The peptides of the cleaved SLPI (cSLPI) remain coupled due to the disulfide bonds in the molecule but under reducing conditions the cleavage can be observed as peptide products. Subsequent ex vivo studies confirmed the presence of SLPI in human saliva and its susceptibility to cleavage by chymase. Furthermore, inhibitors of chymase activity are able to inhibit this cleavage. Human saliva from both normal and allergic individuals was analyzed for levels of cSLPI and a correlation between the level of cSLPI and the extent of allergic symptoms was observed, suggesting the application of cSLPI as a biomarker of chymase activity in humans.

References

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Citations

Oct 31, 2009·American Journal of Respiratory and Critical Care Medicine·Bruce E MaryanoffWilliam M Abraham
Jul 6, 2010·Biochemical Pharmacology·Jukka KervinenLawrence de Garavilla
Apr 30, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S M BelkowskiM R D'Andrea
Apr 19, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Megan Meyer, Ilona Jaspers
Dec 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sinéad WeldonClifford C Taggart
Nov 29, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Megan MeyerIlona Jaspers
Jun 13, 2018·Frontiers in Immunology·Jennifer VandoorenGhislain Opdenakker
Feb 20, 2021·Cytokine & Growth Factor Reviews·Sandrine Nugteren, Janneke N Samsom

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