CLEC10A Is a Specific Marker for Human CD1c+ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion

Frontiers in Immunology
Lukas HegerDiana Dudziak

Abstract

Dendritic cells (DCs) are major players for the induction of immune responses. Apart from plasmacytoid DCs (pDCs), human DCs can be categorized into two types of conventional DCs: CD141+ DCs (cDC1) and CD1c+ DCs (cDC2). Defining uniquely expressed surface markers on human immune cells is not only important for the identification of DC subpopulations but also a prerequisite for harnessing the DC subset-specific potential in immunomodulatory approaches, such as antibody-mediated antigen targeting. Although others identified CLEC9A as a specific endocytic receptor for CD141+ DCs, such a receptor for CD1c+ DCs has not been discovered, yet. By performing transcriptomic and flow cytometric analyses on human DC subpopulations from different lymphohematopoietic tissues, we identified CLEC10A (CD301, macrophage galactose-type C-type lectin) as a specific marker for human CD1c+ DCs. We further demonstrate that CLEC10A rapidly internalizes into human CD1c+ DCs upon binding of a monoclonal antibody directed against CLEC10A. The binding of a CLEC10A-specific bivalent ligand (the MUC-1 peptide glycosylated with N-acetylgalactosamine) is limited to CD1c+ DCs and enhances the cytokine secretion (namely TNFα, IL-8, and IL-10) induced by TLR 7/8...Continue Reading

References

Apr 1, 1998·Nature·J Banchereau, R M Steinman
Mar 17, 2000·Immunological Reviews·R Medzhitov, C Janeway
Sep 19, 2001·The Journal of Experimental Medicine·D HawigerM C Nussenzweig
Mar 17, 2004·The Journal of Experimental Medicine·Laura C BonifazRalph M Steinman
Dec 30, 2006·Cancer Immunology, Immunotherapy : CII·Eirikur SaelandYvette van Kooyk
Jan 6, 2007·Science·Diana DudziakMichel C Nussenzweig
Feb 26, 2008·Nature Immunology·Jonathan C KaganRuslan Medzhitov
May 24, 2008·The Journal of Clinical Investigation·David SanchoCaetano Reis e Sousa
Sep 19, 2008·Immunity·José A Villadangos, Louise Young
Mar 25, 2010·European Journal of Immunology·Olivier P JoffreCaetano Reis e Sousa
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Satwinder Kaur SinghYvette van Kooyk
May 19, 2010·The Journal of Experimental Medicine·Lionel Franz PoulinCaetano Reis e Sousa
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joel N H SternJack L Strominger
Jan 12, 2011·Annual Review of Immunology·Boris ReizisVanja Sisirak
Jul 14, 2012·Nature Reviews. Immunology·Olivier P JoffreSebastian Amigorena
Jan 24, 2013·Journal of Leukocyte Biology·Caroline HémontRégis Josien
Jan 29, 2013·Immunity·Elodie SeguraSebastian Amigorena
Jun 8, 2013·Journal of Leukocyte Biology·Sandra J van VlietYvette van Kooyk

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Citations

May 20, 2020·Chembiochem : a European Journal of Chemical Biology·Pablo ValverdeJesús Jiménez-Barbero
May 7, 2020·Journal of the Royal Society, Interface·Daniel P HowsmonMichael S Sacks
Jun 28, 2020·European Journal of Immunology·G Tuba BarutStephanie C Talker
Jul 12, 2020·International Journal of Molecular Sciences·J Kenneth Hoober
Nov 15, 2018·Frontiers in Immunology·Stephanie C TalkerArtur Summerfield
Aug 1, 2020·Frontiers in Cellular and Infection Microbiology·Malgorzata E MnichNina M van Sorge
Jan 11, 2020·Frontiers in Immunology·J Kenneth HooberRobert Cote
Sep 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Youjin HuWei Chi
Oct 13, 2020·Frontiers in Immunology·Sandra C Silva-CardosoAridaman Pandit
Oct 18, 2019·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Helen HeEmma Guttman-Yassky
Oct 27, 2020·Frontiers in Immunology·Lukas HegerLoems Ziegler-Heitbrock
Apr 2, 2021·Frontiers in Immunology·Ryutaro IwabuchiYoshimasa Takahashi
May 16, 2020·Molecular Immunology·Xin-Long Luo, Marc Dalod
Apr 14, 2021·Immunology Letters·Lukas HatscherDiana Dudziak
Dec 1, 2020·Trends in Immunology·Javiera Villar, Elodie Segura
May 24, 2021·Seminars in Immunology·Ariel E Marciscano, Niroshana Anandasabapathy
Jul 21, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuehan GaoWenru Su
Dec 21, 2021·Molecular Pharmaceutics·François BulteauJean-Luc Coll

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Datasets Mentioned

BETA
GSE77671

Methods Mentioned

BETA
density gradient centrifugation
feature extraction
flow cytometry
FACS

Software Mentioned

Treestar
R
GraphPad Prism
gplots
Phosflow analyses
FlowJo
Phosflow
LEGENDplex

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