Clenbuterol Induces Cell Cycle Arrest in C2C12 Myoblasts by Delaying p27 Degradation through β-arrestin 2 Signaling

International Journal of Biological Sciences
Min ChenQingyong Meng

Abstract

β2-Adrenoceptor (β2-AR) agonists promote muscle growth. The aim of this study was to elucidate some effects of the selective β2-adrenoceptor agonist clenbuterol (CLB) on myoblast proliferation. We found that CLB induces cell cycle arrest in C2C12 myoblasts. This effect is partly due to the enhanced stability of p27, rather than the increased gene transcription via cAMP response element-binding protein (CREB). Specifically, CLB treatment enhanced the accumulation of p27 in the nucleus while depleting it from the cytosol via a mechanism that requires β2-AR. Surprisingly, p27 accumulation was not reversed by the protein kinase A (PKA) inhibitor H-89, but interestingly, was alleviated by the knockdown of β-arrestin 2. Thus, our work provides a basis for β2-AR agonists inhibit myoblasts proliferation through signaling via β2-AR, β-arrestin 2, and p27.

Citations

May 28, 2021·Biochemistry and Biophysics Reports·Boimpoundi Eunice Flavie Ouali, Hao-Ven Wang
Jul 14, 2021·DNA and Cell Biology·Chuncheng LiuQingyong Meng
Sep 18, 2021·Stem Cell Research & Therapy·Shiguo YuanXiaochun Bai

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Methods Mentioned

BETA
transfections
flow cytometry
Assay
transfection

Software Mentioned

SPSS

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