CLIC4 regulates apical exocytosis and renal tube luminogenesis through retromer- and actin-mediated endocytic trafficking

Nature Communications
Szu-Yi ChouChing-Hwa Sung

Abstract

Chloride intracellular channel 4 (CLIC4) is a mammalian homologue of EXC-4 whose mutation is associated with cystic excretory canals in nematodes. Here we show that CLIC4-null mouse embryos exhibit impaired renal tubulogenesis. In both developing and developed kidneys, CLIC4 is specifically enriched in the proximal tubule epithelial cells, in which CLIC4 is important for luminal delivery, microvillus morphogenesis, and endolysosomal biogenesis. Adult CLIC4-null proximal tubules display aberrant dilation. In MDCK 3D cultures, CLIC4 is expressed on early endosome, recycling endosome and apical transport carriers before reaching its steady-state apical membrane localization in mature lumen. CLIC4 suppression causes impaired apical vesicle coalescence and central lumen formation, a phenotype that can be rescued by Rab8 and Cdc42. Furthermore, we show that retromer- and branched actin-mediated trafficking on early endosome regulates apical delivery during early luminogenesis. CLIC4 selectively modulates retromer-mediated apical transport by negatively regulating the formation of branched actin on early endosomes.

References

May 3, 1991·Cell·W StoorvogelA L Schwartz
Mar 1, 1985·Kidney International·L C RacusenK Solez
Mar 27, 2001·Current Biology : CB·A M WeaverJ A Cooper
Oct 19, 2001·Oncogene·S A Weed, J T Parsons
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eunkyung Lee, Pietro De Camilli
May 8, 2002·Nature Reviews. Molecular Cell Biology·Erik Ilsø Christensen, Henrik Birn
Oct 4, 2002·Current Biology : CB·Antje Gohla, Gary M Bokoch
Apr 1, 1955·The American Journal of Physiology·G FALK
Mar 3, 2004·Biochemistry·Kyoungtae KimJohn A Cooper
Apr 14, 2004·The Journal of Cell Biology·Cecilia N ArighiJuan S Bonifacino
Jun 5, 2004·Developmental Cell·Verena GöbelJohn T Fleming
Jul 13, 2004·Nature Cell Biology·Marcel VergésKeith E Mostov
Jan 1, 2005·American Journal of Human Genetics·Richard R HoopesSteven J Scheinman
May 19, 2006·Journal of the American Society of Nephrology : JASN·Prasad Devarajan
May 29, 2007·Journal of Inherited Metabolic Disease·M A BenningaB T Poll-The
Sep 4, 2007·Developmental Cell·Kai S ErdmannPietro De Camilli
Sep 25, 2007·Nature·Aitor HierroJames H Hurley
Jul 8, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Leo Monnens, Elena Levtchenko
Oct 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Puneet KhandelwalGerard Apodaca
Sep 25, 2009·Molecular Biology of the Cell·Bas PonsioenWouter H Moolenaar
Nov 20, 2009·Developmental Cell·Timothy S Gomez, Daniel D Billadeau
Nov 20, 2009·Developmental Cell·Emmanuel DeriveryAlexis Gautreau
Nov 20, 2009·Journal of Cell Science·Ora A Weisz, Enrique Rodriguez-Boulan
Jan 6, 2010·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Rikke Nielsen, Erik Ilsø Christensen
Mar 20, 2010·Nature Reviews. Molecular Cell Biology·Kenneth G Campellone, Matthew D Welch
May 18, 2010·The Journal of Biological Chemistry·Puneet GargLawrence B Holzman

❮ Previous
Next ❯

Citations

Aug 9, 2016·Annual Review of Cell and Developmental Biology·Antonio Castro-CastroPhilippe Chavrier
Nov 5, 2016·Annual Review of Physiology·Megan L Eshbach, Ora A Weisz
Nov 18, 2016·Journal of Cell Science·Elisabetta Argenzio, Wouter H Moolenaar
Nov 2, 2018·The Journal of Biological Chemistry·Elisabetta ArgenzioMetello Innocenti
Nov 30, 2018·Nature Communications·Álvaro Román-FernándezDavid M Bryant
Nov 3, 2016·American Journal of Physiology. Renal Physiology·Mahtab TavasoliBarbara J Ballermann
Dec 28, 2019·Life Science Alliance·Zeynep Cansu Uretmen KagialiNurhan Ozlu
Mar 4, 2020·Development·Chii J Chan, Takashi Hiiragi
Mar 10, 2017·Physiological Reports·Barbara UlmasovJohn C Edwards
Aug 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yuji MizotaniMasaya Imoto
Sep 11, 2020·Journal of Developmental Biology·Matthew BuechnerHikmat Al-Hashimi
Jul 20, 2021·Molecular Medicine Reports·Hui WangBiguang Tuo
Nov 29, 2021·Alcoholism, Clinical and Experimental Research·James W BogenpohlMichael F Miles

❮ Previous
Next ❯

Methods Mentioned

BETA
co-immunoprecipitation
pull down
transfection
nucleotide exchange
GTPases
GTPase
immunoprecipitation
immunoprecipitation assay

Software Mentioned

Image J
Coloc2
Deconvolution
Photoshop
Adobe

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.