Click chemistry inspired copper sulphide nanoparticle-based fluorescence assay of kanamycin using DNA aptamer

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Ahmed S F BelalTarek S Belal

Abstract

A highly selective and sensitive fluorescence assay for kanamycin has been developed that depends on complementation of two splits of DNA aptamer. One DNA split was labeled with CuS nanoparticle and the other was decorated with biotin, which enabled coupling with streptavidin magnesphere paramagnetic particles (PMPs). Complementation of the two-aptamer splits happened only in the presence of kanamycin and the subsequent sandwich was separated via a magnet. The released Cu(II) was reduced to Cu(I) by sodium ascorbate and finally catalyzed the click reaction between fluorogenic 3-azido-7-hydroxycoumarin and propargyl alcohol to afford the corresponding fluorescent 1,4-disubstituted-1,2,3-triazole. The fluorescence signal produced (λex. = 365 nm, λem. = 470 nm) was dependent on kanamycin concentration. Fluorescence signal amplification was found to be in good linear relationship with the logarithm of kanamycin concentration in the range of 0.04-20 nM. Furthermore, the proposed assay showed a good reproducibility, high selectivity and low detection limits for kanamycin determination. In addition, the capability of the proposed method to detect kanamycin in biological samples with satisfactory results was demonstrated.

Citations

Jul 13, 2019·Critical Reviews in Food Science and Nutrition·Wei WuQingli Yang
Jul 15, 2020·Critical Reviews in Analytical Chemistry·Pei WangJuan Zhang
Mar 1, 2020·Nucleic Acids Research·Mégane DebiaisSabine Müller
Oct 31, 2020·The Science of the Total Environment·Fengling YueXia Sun
Mar 28, 2021·Journal of Separation Science·Md Musfiqur RahmanA M Abd El-Aty
Jul 7, 2021·Analytical Methods : Advancing Methods and Applications·Jiaxin ZhangLongshan Zhao
Jul 17, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yunlian ZhangQuansheng Chen

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