"Click" immobilization of a VEGF-mimetic peptide on decellularized endothelial extracellular matrix to enhance angiogenesis

ACS Applied Materials & Interfaces
Lin WangChengzhi Cai


We show that coating of decellularized extracellular matrix (DC-ECM) on substrate surfaces is an efficient way to generate a platform mimicking the native ECM environment. Moreover, the DC-ECM can be modified with a peptide (QK) mimicking vascular endothelial growth factor without apparently compromising its integrity. The modification was achieved through metabolic incorporation of a "clickable" handle to DC-ECM followed by rapid attachment of the QK peptide with an azido tag using copper-catalyzed click reaction. The attachment of the QK peptide on to DC-ECM in this way further enhanced the angiogenic responses (formation of branched tubular networks) of endothelial cells.


Sep 23, 2000·Nature·G D YancopoulosJ Holash
Jul 31, 2003·Journal of Biomedical Materials Research. Part a·Minh T PhamManfred F Maitz
Dec 16, 2004·Angewandte Chemie·Lei Wang, Peter G Schultz
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Luca Domenico D'AndreaCarlo Pedone
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Néha DattaAntonios G Mikos
Oct 13, 2006·Angewandte Chemie·Kimberly E BeattyDavid A Tirrell
Apr 9, 2008·Current Topics in Medicinal Chemistry·James J Moon, Jennifer L West
May 6, 2008·Tissue Engineering. Part B, Reviews·Megan E FrancisEric M Brey
Dec 2, 2008·Journal of Cellular and Molecular Medicine·Dan LiaoChangyi Chen
May 22, 2009·Nature Materials·Elsie S PlaceMolly M Stevens
Nov 17, 2009·Journal of the American Chemical Society·Sophia W LiaoZhibin Guan
Dec 8, 2009·Journal of the American Chemical Society·John T WilsonElliot L Chaikof
Jan 14, 2010·Journal of the American Chemical Society·Ratmir DerdaLaura L Kiessling
Feb 18, 2010·Journal of the American Chemical Society·Kerstin M GallerJeffrey D Hartgerink
Jun 26, 2010·Science·Thomas H PetersenLaura E Niklason
Jul 16, 2010·Nature Medicine·Harald C OttJoseph P Vacanti
Jul 31, 2010·Science·William R Wagner, Bartley P Griffith
Sep 16, 2010·Journal of the American Chemical Society·Bo LiuWei Shen
Oct 1, 2010·Advanced Materials·Lisa E FreedFarshid Guilak
Nov 11, 2010·Journal of the American Chemical Society·David Soriano Del AmoPeng Wu
Dec 15, 2010·Nature Nanotechnology·Tal DvirRobert Langer
Feb 8, 2011·Biomaterials·Peter M CrapoS F Badylak
Mar 15, 2011·Advanced Drug Delivery Reviews·Esther C NovoselPetra J Kluger
May 13, 2011·Journal of the American Chemical Society·Debjit DuttaMuhammad N Yousaf
Sep 24, 2011·Nature Materials·Jennifer L West


Feb 15, 2019·Journal of Materials Science. Materials in Medicine·Tatjana FloraJ Carlos Rodríguez-Cabello
May 28, 2016·Annals of Biomedical Engineering·Dylan J RichardsYing Mei
Jan 18, 2018·Journal of Biomaterials Applications·Liancheng XuZhiwei Wang
Jan 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wang LinWang Yingjun
Mar 30, 2017·Chemical Science·Siheng LiChengzhi Cai
Sep 13, 2020·Nanomaterials·Sara PolitiLaura Teodori
Jan 5, 2021·Molecular Biology Reports·Nicolas Pavlos OmorphosMiljyot Singh Sangha

Methods Mentioned


Related Concepts

Extracellular Matrix
Growth Factor
Endothelial Cells
Angiogenic Process
Tubular Cells
Vascular Endothelial Cells

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