We show that coating of decellularized extracellular matrix (DC-ECM) on substrate surfaces is an efficient way to generate a platform mimicking the native ECM environment. Moreover, the DC-ECM can be modified with a peptide (QK) mimicking vascular endothelial growth factor without apparently compromising its integrity. The modification was achieved through metabolic incorporation of a "clickable" handle to DC-ECM followed by rapid attachment of the QK peptide with an azido tag using copper-catalyzed click reaction. The attachment of the QK peptide on to DC-ECM in this way further enhanced the angiogenic responses (formation of branched tubular networks) of endothelial cells.
Effect of bone extracellular matrix synthesized in vitro on the osteoblastic differentiation of marrow stromal cells
Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering
Targeting angiogenesis: structural characterization and biological properties of a de novo engineered VEGF mimicking peptide
In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation
Covalent linkage of heparin provides a stable anti-coagulation surface of decellularized porcine arteries.
The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art
Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues
Self-assembling multidomain peptide hydrogels: designed susceptibility to enzymatic cleavage allows enhanced cell migration and spreading
Synthetic chemoselective rewiring of cell surfaces: generation of three-dimensional tissue structures
The promotion of microvasculature formation in poly(ethylene glycol) diacrylate hydrogels by an immobilized VEGF-mimetic peptide
The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching
Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels
Cell-Free Hydrogel System Based on a Tissue-Specific Extracellular Matrix for In Situ Adipose Tissue Regeneration
Multifunctional hybrid nanoconstructs facilitate intracellular localization of doxorubicin and genistein to enhance apoptotic and anti-angiogenic efficacy in breast adenocarcinoma
Constructing heparin-modified pancreatic decellularized scaffold to improve its re-endothelialization
Multi-biofunctionalization of a titanium surface with a mixture of peptides to achieve excellent antimicrobial activity and biocompatibility
Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications-A Review
Understanding angiogenesis and the role of angiogenic growth factors in the vascularisation of engineered tissues.
Arterial-Venous in Development & Disease
Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.