Clindamycin uptake by human neutrophils

The Journal of Infectious Diseases
M S Klempner, B Styrt

Abstract

An anaerobic environment limits the microbicidal capacity of polymorphonuclear neutrophils (PMNs). To augment PMN killing under these conditions, the characteristics and mechanisms of clindamycin uptake by human PMNs were studied. The peak intracellular concentration of clindamycin was approximately 40 times greater than the extracellular concentration. Clindamycin uptake was rapid, saturable, and temperature-dependent. Intracellular accumulation of the drug was inhibited in acid pH, and agents that collapsed the transmembrane pH gradients also inhibited uptake. Isolated PMN lysosomes also accumulated clindamycin against a large concentration gradient, and uptake was reduced by collapsing the translysosomal membrane pH gradient. The intracellular drug was fully bioactive. These studies demonstrate that clindamycin is rapidly accumulated by PMNs, that drug uptake is related to a pH gradient, and that clindamycin appears to be lysosomotropic.

Citations

Feb 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P M Tulkens
Dec 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I GarcíaE J Perea
Nov 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A PascualE J Perea
Apr 1, 1987·European Journal of Clinical Microbiology·A PascualP Peterson
Dec 1, 1982·European Journal of Clinical Microbiology·P K Peterson
Mar 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I GarcíaE J Perea
Feb 1, 1995·Comparative Immunology, Microbiology and Infectious Diseases·M I PedreraA B Rodriguez
Feb 1, 1986·Diagnostic Microbiology and Infectious Disease·J L Ho, M S Klempner
Jul 1, 1992·Diagnostic Microbiology and Infectious Disease·A PascualE J Perea
Dec 9, 2003·International Journal of Antimicrobial Agents·Iris H HallTimothy Ives
Aug 5, 2000·International Journal of Antimicrobial Agents·I GarcíaE J Perea
Nov 15, 2002·International Journal of Antimicrobial Agents·Iris H HallTimothy J Ives
Jun 1, 1997·International Journal of Antimicrobial Agents·M Maurin, D Raoult
May 13, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sandrine LemairePaul M Tulkens
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Isabel GarcíaAlvaro Pascual
Oct 1, 1988·Antimicrobial Agents and Chemotherapy·P J Baker, M E Wilson
May 1, 1989·Antimicrobial Agents and Chemotherapy·A PascualE J Perea
Jun 1, 1990·Antimicrobial Agents and Chemotherapy·W L Hand, N L King-Thompson
Jul 1, 1991·Antimicrobial Agents and Chemotherapy·J el BennaM T Labro
Jun 1, 1992·Antimicrobial Agents and Chemotherapy·D R BaldwinR Wise
Dec 1, 1993·Antimicrobial Agents and Chemotherapy·J BlaisS Chamberland
Feb 1, 1993·Antimicrobial Agents and Chemotherapy·A PascualE J Perea
Jan 1, 1994·Antimicrobial Agents and Chemotherapy·E R Pfefferkorn, S E Borotz
Oct 1, 1994·Antimicrobial Agents and Chemotherapy·I GarcíaE J Perea
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·I GarcíaE J Perea
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Alvaro PascualEvelio J Perea
Jul 1, 1985·The Journal of Clinical Investigation·M S Klemper
Feb 22, 2002·Japanese Journal of Pharmacology·Hiroshi YamamotoKeishi Kubo
Mar 3, 2004·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Iris H HallTimothy J Ives
Jan 1, 1988·Critical Reviews in Microbiology·C E SwensonR S Ginsberg
Apr 15, 1985·American Journal of Obstetrics and Gynecology·D V Landers, R L Sweet
Nov 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·G Gillissen
Sep 3, 1999·Mayo Clinic Proceedings·M J Kasten
Dec 14, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elsa BrionesJosé M Lanao
Apr 6, 1992·The American Journal of Medicine·E J PereaA Pascual

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