Clinical application and histological properties of autologous tissue-engineered skin equivalents using an acellular dermal matrix

Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi
Yoshihiro TakamiHiko Hyakusoku

Abstract

We developed a transplantable tissue-engineered skin equivalent composed of autologous cultured keratinocytes, fibroblasts, and a decellularized allogeneic dermis (acellular allogeneic dermal matrix; ADM) obtained from cadavers. In a process taking 3 weeks, cultured autologous keratinocytes from burn patients were expanded and then grown on ADMs. The tissue-engineered autologous skin equivalents (TESEs) were then transplanted in a one-stage procedure to the debrided third-degree burn wounds of 4 patients. The mean graft survival rate was 96%. Delayed graft loss and graft fragility were not observed. Histological and immunohistological findings indicated that the transplanted TESE had similar characteristics to normal human split-thickness skin grafts. These results suggest that the TESE using ADM can be used for permanent repair of full-thickness skin defects.

References

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Citations

Mar 25, 2019·Journal of Biomedical Materials Research. Part a·Sandra StrassburgGünter Finkenzeller
Sep 29, 2018·The Journal of Dermatological Treatment·Christina DaiAmor Khachemoune
Feb 15, 2020·Nature Reviews. Disease Primers·Marc G JeschkeSarvesh Logsetty
Sep 10, 2016·Tissue Engineering. Part B, Reviews·Dorothée GirardAlexis Desmoulière
Jun 9, 2017·Experimental Biology and Medicine·H E AbaciAngela Christiano
Jan 27, 2021·Journal of Clinical Medicine·Anna Paulina Domaszewska-SzostekMaria Siemionow
Mar 11, 2021·International Journal of Surgery Case Reports·Frederica Jessie Tchoungui RitzAntonio Iannelli
Jun 19, 2021·Npj Regenerative Medicine·Álvaro Sierra-SánchezSalvador Arias-Santiago

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