PMID: 9431938Feb 12, 1998Paper

Clinical application of PCR-restriction enzyme pattern analysis for rapid identification of aerobic actinomycete isolates

Journal of Clinical Microbiology
R W WilsonRichard J Wallace

Abstract

The accuracy and practicality of PCR-restriction enzyme pattern analysis (PRA) for routine identification of aerobic actinomycete clinical isolates were evaluated for 299 cultures submitted to the Mycobacteria/Nocardia Laboratory at the University of Texas Health Center at Tyler. PRA identification using an amplified 439-bp segment (amplicon) of the 65-kDa heat shock protein gene was compared to identification by traditional methods, including growth characteristics, susceptibility patterns, biochemical testing, and high-performance liquid chromatography analysis. Microbiological examination of six cultures ruled out aerobic actinomycetes, and they were omitted from the study. Amplicons were analyzed with BstEII, HaeIII, MspI, HinfI, and BsaHI. When necessary, AciI, HhaI, and NarI were also used. From March 1995 through May 1997 (27 months), 274 of the remaining 293 (93.5%) isolates were accurately identified by PRA. Major diagnostic groups included 170 mycobacteria, 93 nocardiae, and 30 other aerobic actinomycetes. Mixed cultures were readily recognized by PRA, including a wound culture that contained two Nocardia taxa that were indistinguishable morphologically. Mycobacterium mucogenicum was identified in three cultures heavi...Continue Reading

References

Sep 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M M McNeilZ M Blacklock
May 18, 1992·The Medical Journal of Australia·P R Georghiou, Z M Blacklock
Nov 11, 1991·Journal of Clinical Microbiology·R J WallaceG O Onyi
Dec 1, 1988·Antimicrobial Agents and Chemotherapy·R J WallaceJ M Smith
Jul 1, 1988·The Journal of Applied Bacteriology·C H CollinsA H Uttley
Mar 1, 1988·Diagnostic Microbiology and Infectious Disease·R J Wallace, L C Steele
Jun 1, 1980·Journal of Clinical Microbiology·S K MishraD A Barnett
Jun 1, 1995·Journal of Clinical Microbiology·R J WallaceJ Gibson
Sep 1, 1993·Antimicrobial Agents and Chemotherapy·V A SteingrubeD R Nash

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Citations

Jan 28, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·N M Clark, UNKNOWN AST Infectious Diseases Community of Practice
Aug 21, 2012·Critical Reviews in Microbiology·Kavita Tiwari, Rajinder K Gupta
Mar 8, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·N M ClarkUNKNOWN AST Infectious Diseases Community of Practice
Jan 1, 2014·International Scholarly Research Notices·Muhammad KhanSufyan Abdul Mujeeb
Oct 27, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Mar 1, 2019·Clinical Transplantation·Alejandro RestrepoUNKNOWN Infectious Diseases Community of Practice of the American Society of Transplantation
May 18, 2007·Journal of Clinical Microbiology·Sadao JinnoRussell Wong
Dec 8, 2004·Journal of Clinical Microbiology·Yansheng ZhangRichard J Wallace
Oct 30, 2001·Journal of Clinical Microbiology·M A YakrusB Metchock
Jun 9, 2004·Journal of Clinical Microbiology·Jean Baldus PatelIrving Nachamkin
Oct 9, 2003·Journal of Clinical Microbiology·Michael A Saubolle, Den Sussland
Apr 12, 2003·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Fred A LopezMark Holodniy
Feb 13, 1999·Journal of Clinical Microbiology·H RinguetC Pierre-Audigier
Dec 17, 1998·Journal of Clinical Microbiology·F J LaurentP Boiron
Aug 1, 2002·Journal of Clinical Microbiology·Richard J WallaceKenneth C Jost
Jun 14, 2005·Archives of Medical Research·José Francisco Valenzuela-TovarHiram Olivera-Díaz

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