Clinical application of targeted next-generation sequencing for colorectal cancer patients: a multicentric Belgian experience

Oncotarget
Nicky D'HaeneMarie Le Mercier

Abstract

International guidelines made RAS (KRAS and NRAS) status a prerequisite for the use of anti-EGFR agents for metastatic colorectal cancer (CRC) patients. Daily, new data emerges on the theranostic and prognostic role of molecular biomarkers; this is a strong incentive for a validated, sensitive, and broadly available molecular screening test. Next-generation sequencing (NGS) has begun to supplant other technologies for genomic profiling. We report here our 2 years of clinical practice using NGS results to guide therapeutic decisions. The Ion Torrent AmpliSeq colon/lung cancer panel, which allows mutation detection in 22 cancer-related genes, was prospectively used in clinical practice (BELAC ISO 15189 accredited method). The DNA of 741 formalin-fixed paraffin-embedded CRC tissues, including primary tumors and metastasis, was obtained from 14 different Belgian institutions and subjected to targeted NGS. Of the tumors tested, 98% (727) were successfully sequenced and 89% (650) harbored at least one mutation. KRAS, BRAF and NRAS mutations were found in 335 (46%), 78 (11%) and 32 (4%) samples, respectively. These mutation frequencies were consistent with those reported in public databases. Moreover, mutations and amplifications in p...Continue Reading

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Citations

Nov 28, 2018·International Journal of Molecular Sciences·Chiara MolinariPaola Ulivi
Aug 30, 2020·Biomedicines·Marco VacanteAntonio Biondi
Jul 11, 2019·Diagnostic Cytopathology·Kelsey E McHughJordan P Reynolds
Oct 1, 2020·Computational and Structural Biotechnology Journal·Zodwa DlaminiRahaba Marima
Aug 19, 2020·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Youngwoo LeeYeul Hong Kim
Aug 24, 2019·Translational Oncology·Dohee KwonKyoung-Mee Kim
Apr 16, 2021·BMC Medical Genomics·Mustafa ÖzdoğanGeorge Nasioulas

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Software Mentioned

torrent suite
Integrative Genome Viewer ( IGV )
Variant Caller

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