Clinical applications of luminescent assays for enzymes and enzyme labels

Journal of Clinical Laboratory Analysis
I Bronstein, L J Kricka

Abstract

A wide range of luminescent assays for enzyme labels has been developed. The clinical application of luminescent assays for horseradish peroxidase, alkaline phosphatase, xanthine oxidase, beta-D galactosidase, and glucose 6-phosphate dehydrogenase is discussed. The enzyme luminescent methods provide improved sensitivity compared with colorimetric and fluorimetric assays. A new reagent, dioxetane phosphate, has provided a simple and extremely sensitive assay for alkaline phosphatase. The available instruments and new photographic detection techniques for luminescent assays are briefly discussed.

References

Sep 1, 1979·Journal of Clinical Microbiology·R H Yolken, P J Stopa
Apr 1, 1986·Analytical Biochemistry·B TérouanneA Crastes de Paulet
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·I BronsteinJ C Voyta
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·J M ClyneM S Urdea
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·W Miska, R Geiger
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·A Baret, V Fert
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·S B VlasenkoA M Yegorov
Jun 1, 1988·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·S OzawaK Yanagi
Feb 1, 1987·European Journal of Clinical Microbiology·R Schneppenheim, P Rautenberg
Nov 15, 1988·Analytical Biochemistry·L J Kricka
Feb 11, 1988·Nucleic Acids Research·R Hauber, R Geiger
Apr 1, 1986·Analytical Biochemistry·B TérouanneA Crastes de Paulet
Apr 1, 1986·Analytical Biochemistry·M L CarriéA Crastes de Paulet
Jan 1, 1986·Methods in Enzymology·G H Thorpe, L J Kricka
Nov 15, 1985·Analytical Biochemistry·J A MatthewsL J Kricka
May 15, 1982·Analytical Biochemistry·J Wannlund, M DeLuca
Dec 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·B ROTMAN

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Citations

Apr 8, 1991·Journal of Immunological Methods·E Roffman, N Frenkel
Jul 1, 1992·Journal of Virological Methods·M MusianiM La Placa
Jun 30, 1994·Journal of Biotechnology·C Kessler
Feb 1, 1991·Current Opinion in Biotechnology·J C Richards
Jan 1, 1995·Journal of Viral Hepatitis·G L Ornopia, K Kuramoto
Mar 6, 2015·Protein Science : a Publication of the Protein Society·Kotaro NishiyamaTetsuya Yomo
Nov 1, 1990·Analytical Chemistry·S Beck, H Köster
Apr 4, 2018·Cold Spring Harbor Protocols·Larry J Kricka
Oct 1, 1991·Journal of Bioluminescence and Chemiluminescence·L J KrickaB Edwards
Jun 24, 2005·Nature Methods·Tom S WehrmanHelen M Blau
Jul 9, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·L J KrickaP E Stanley
Jul 9, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·L J KrickaP E Stanley
May 14, 2013·Journal of Chemical Theory and Computation·Ling Yue, Ya-Jun Liu

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