Clinical associations between acetylcholine levels and cholinesterase activity in saliva and gingival crevicular fluid and periodontal diseases

Journal of Clinical Periodontology
Danae A ApatzidouChristopher J Nile

Abstract

The oral mucosa possesses a non-neuronal cholinergic system. This study aimed to determine clinical evidence for a role of cholinergic mechanisms in the pathogenesis of periodontal diseases. Fifty healthy participants, 52 patients with gingivitis and 49 with periodontitis were recruited. Full periodontal parameters were recorded and saliva and gingival crevicular fluid (GCF) collected. Levels of acetylcholine and inflammatory mediators were quantified using commercially available assay kits. Acetylcholinesterase and butyrylcholinesterase activities were measured using a published biochemical assay. Acetylcholine levels are significantly elevated in saliva and GCF, whereas GCF levels of butyrylcholinesterase activity are significantly decreased, in patients with periodontal diseases. Acetylcholine levels in saliva and GCF correlated positively with clinical markers of disease severity and with increased levels of IL-17A and IL-17F. In contrast, butyrylcholinesterase activity levels in GCF showed significant negative correlations with clinical markers of disease severity and IL-17A and IL-17F levels. None of the findings were due to smoking. Elevated acetylcholine levels and reduced butyrylcholinesterase activity are clinically a...Continue Reading

References

Sep 20, 1993·Annals of the New York Academy of Sciences·M Navazesh
Jul 1, 1993·The Journal of Investigative Dermatology·S A GrandoM V Dahl
Apr 1, 1997·Naunyn-Schmiedeberg's Archives of Pharmacology·H KlapprothI Wessler
Jan 18, 2003·Analytical Biochemistry·Peter EyerElsa Reiner
Feb 4, 2003·Nature Reviews. Neuroscience·Sultan DarveshChangiz Geula
Mar 12, 2003·Life Sciences·Ignaz WesslerCharles James Kirkpatrick
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Koichiro Kawashima, Takeshi Fujii
Sep 1, 2007·Respiratory Medicine·Frank BühlingTobias Welte
May 27, 2008·British Journal of Pharmacology·I Wessler, C J Kirkpatrick
Jul 17, 2008·The Journal of Infectious Diseases·Ilona A J GiebelenTom van der Poll
Oct 3, 2008·The European Respiratory Journal·I A J GiebelenT van der Poll
Jan 8, 2009·Physiological Reviews·Edson X AlbuquerqueScott W Rogers
Jun 1, 2010·Autonomic Neuroscience : Basic & Clinical·Obaida R RanaErol Saygili
Jan 11, 2011·Journal of Neurochemistry·David HicksAnthony J Turner
Sep 17, 2011·Science·Mauricio Rosas-BallinaKevin J Tracey
Jul 11, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Noha ZoheirChristopher J Nile
Dec 4, 2012·International Journal of Molecular Sciences·Marcella RealeAda Maria Tata
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Colin ReardonTak W Mak
Feb 12, 2013·The European Respiratory Journal·Loes E M KistemakerReinoud Gosens
Dec 4, 2013·Pharmacology·Janet Beckmann, Katrin Susanne Lips
Feb 14, 2014·Stroke; a Journal of Cerebral Circulation·Johanna RuhnauAntje Vogelgesang
Mar 13, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Alexandrea MacphersonChristopher J Nile
Nov 6, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Raja Azman AwangChristopher Nile
May 14, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Leon P McLeanTerez Shea-Donohue
Nov 4, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shobhit DhawanWouter J de Jonge
Dec 7, 2016·Journal of Inflammation Research·Aleksandar R ZivkovicKarsten Schmidt
Sep 22, 2017·Frontiers in Immunology·Takeshi FujiiKoichiro Kawashima
Nov 29, 2017·The Journal of Surgical Research·Aleksandar R ZivkovicKarsten Schmidt

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Citations

Nov 16, 2019·The Journal of Experimental Medicine·Immo PrinzUlrich Mrowietz
Nov 5, 2020·Cytokine·Nidhi MedaraNeil M O'Brien-Simpson
Jan 2, 2021·Research in Veterinary Science·Meriç KocatürkZeki Yilmaz
May 4, 2021·Frontiers in Immunology·Namrita Halder, Girdhari Lal
Aug 20, 2021·Therapeutic Advances in Chronic Disease·Helena Iznardo, Lluís Puig

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