PMID: 698919Sep 1, 1978Paper

Clinical bladder cancer in sponge matrix tissue culture: procedures for collection, cultivation, and assessment of viability

Cancer
N A AbazaB A Zajac

Abstract

We studied the growth of surgically resected human bladder cancer in three dimensional tissue culture, Observations were made in the living cultures and after the sponge matrix cultures were fixed and prepared as histologic sections. We compared the histologic appearance of each tumor immediately after removal and after a week in culture. In a few instances the donor tumor was kept in medium at room temperature for one day before being inoculated into the matrix. This delay had only slight deleterious effect on the growth in culture. Over 85 consecutive cases have been placed in culture and the first 47 have been evaluated in histologic preparations. Tumors grew into the matrix with distinctly different patterns even when the zero time specimens were of similar histopathology. We also studied several antibiotics to idenify conditions that would suppress the usual urinary bacterial contaminants without producing cytotoxicity to tumor cells by microscopic evaluation. A combination of gentamicin and kanamycin, each at a concentration of 50 microgram/ml was effective.

References

May 1, 1973·International Journal of Cancer. Journal International Du Cancer·J BubeníkJ Donner
Nov 1, 1974·Journal of the National Cancer Institute·A Y ElliottE E Fraley
Feb 1, 1968·Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology·T Yajima
Nov 1, 1961·The Journal of Urology·R U BREGMAN, E T BREGMAN

Citations

Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A E Freeman, R M Hoffman
May 1, 1986·The Journal of Urology·A H LawsonJ P Weaver

Related Concepts

Malignant Neoplasm of Urinary Bladder
Gentacycol
Kantrex
Neoplasms, Experimental
Culture Techniques

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