Clinical comparison of QUANTA Flash dsDNA chemiluminescent immunoassay with four current assays for the detection of anti-dsDNA autoantibodies

Journal of Immunology Research
Maria InfantinoM Mahler

Abstract

The objective of the present study was to compare QUANTA Flash dsDNA, a chemiluminescent immunoassay (CIA) on the BIO-FLASH, a rapid-response chemiluminescent analyzer, to three other anti-dsDNA antibody assays and to Crithidia luciliae indirect immunofluorescence test (CLIFT). In the first part of the study, 161 samples, 61 from patients suffering from systemic lupus erythematosus (SLE) and 100 from a disease control group, were tested by QUANTA Flash dsDNA CIA, QUANTA Lite dsDNA SC ELISA, BioPlex 2200 multiplex flow immunoassay (MFI), ImmuLisa dsDNA ELISA, and NOVA Lite CLIFT. A second cohort of 69 SLE patients was then tested by QUANTA Flash dsDNA and CLIFT to expand the study. The overall qualitative agreements varied between 77.0% (NOVA Lite CLIFT versus QUANTA Lite) and 89.4% (ImmuLisa versus NOVA Lite CLIFT). The clinical sensitivities for the anti-dsDNA antibody tests varied from 8.2% (NOVA Lite CLIFT) to 54.1% (QUANTA Lite), while the clinical specificities varied from 88.0% (BioPlex 2200) to 100.0% (NOVA Lite CLIFT). Good correlation was found between QUANTA Flash dsDNA and NOVA Lite CLIFT. Significant variations among dsDNA methods were observed. QUANTA Flash dsDNA provides a good combination of sensitivity and speci...Continue Reading

References

Jun 1, 1987·Annals of the Rheumatic Diseases·D A IsenbergA Todd-Pokropek
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Feb 15, 2007·Nature Clinical Practice. Rheumatology·Michael Mahler, Marvin J Fritzler
Oct 20, 2009·Journal of Immunological Methods·John G HanlyKathleen Wilton
Feb 12, 2010·Annals of the New York Academy of Sciences·Michael Mahler, Marvin J Fritzler
Jan 24, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·M MahlerR A Sinico
Jun 4, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chelsea BentowMichael Mahler
Jan 1, 2014·Clinical & Developmental Immunology·M InfantinoM Mahler

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Citations

Nov 4, 2015·Immunopharmacology and Immunotoxicology·Michael MahlerMarvin J Fritzler
Feb 28, 2016·Journal of Immunological Methods·Gabriella LakosThierry Dervieux
Jul 3, 2016·Clinical Reviews in Allergy & Immunology·Mandy SowaDirk Roggenbuck
Jun 26, 2017·Auto- Immunity Highlights·Luigi CinquantaNicola Bizzaro
Apr 11, 2018·Frontiers in Immunology·Kevin DidierAmelie Servettaz

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Methods Mentioned

BETA
ELISA
enzyme linked immunosorbent assays
ELISAs

Software Mentioned

BioPlex
ImmuLisa
BIO
it
NOVA
QUANTA Lite
Analyse
FLASH
QUANTA Flash CIA
CLIFT

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