Clinical development of a VAR2CSA-based placental malaria vaccine PAMVAC: Quantifying vaccine antigen-specific memory B & T cell activity in Beninese primigravidae

Vaccine
Komi GbédandéMulti-centre research paper

Abstract

The antigen VAR2CSA plays a pivotal role in the pathophysiology of pregnancy-associated malaria (PAM) caused by Plasmodium falciparum. A VAR2CSA-based vaccine candidate, PAMVAC, is under development by an EU-funded multi-country consortium (PlacMalVac project). As part of PAMVAC's clinical development, we quantified naturally acquired vaccine antigen-specific memory B and T cell responses in Beninese primigravidae recruited at the beginning of pregnancy and followed up to delivery and beyond. Clinical and parasitological histories were compiled from monthly clinic visits. On 4 occasions (first and fifth month of pregnancy, delivery, 6months post-delivery) peripheral blood mononuclear cells were isolated for in vitro assays. PAMVAC-specific memory B cells as well as those specific for a PAM unrelated P. falciparum antigen (PfEMP1-CIDR1a) and for tetanus toxoid were quantified by ELISpot. Memory T cell responses were assessed by quantifying cytokines (IL-5, IL-6, IL-10, IL-13, IFN-γ, TNF-α) in supernatants of cells stimulated in vitro either with PAMVAC, or mitogen (PHA). Both tetanus toxoid- and PAMVAC-specific memory B cell frequencies increased to reach peak levels in the 5th month and at delivery, respectively and persisted p...Continue Reading

Citations

Jan 13, 2018·The Journal of Infectious Diseases·Manfred AccrombessiValérie Briand
Sep 9, 2018·Clinical and Experimental Immunology·C Álvarez-LarrottaE Arango
Jan 11, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Benjamin MordmüllerMorten A Nielsen
Apr 2, 2019·Frontiers in Endocrinology·Johanna SeitzUdo Rudolf Markert
Dec 13, 2019·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·Wolfram Gottfried MetzgerCarsten Köhler
Mar 28, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cornélia P A HounkonnouGilles Cottrell
Jan 22, 2021·Expert Review of Vaccines·Justin Yai Alamou DoritchamouNicaise Tuikue Ndam

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