Clinical diagnosis of early dengue infection by novel one-step multiplex real-time RT-PCR targeting NS1 gene

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Je-Hyoung KimHyun Park

Abstract

Dengue is a mosquito-borne disease that causes a public health problem in tropical and subtropical countries. Current immunological diagnostics based on IgM and/or nonstructural protein 1 (NS1) antigen are limited for acute dengue infection due to low sensitivity and accuracy. This study aimed to develop a one-step multiplex real-time RT-PCR assay showing higher sensitivity and accuracy than previous approaches. Serotype-specific primers and probes were designed through the multiple alignment of NS1 gene. The linearity and limit of detection (LOD) of the assay were determined. The assay was clinically validated with an evaluation panel that was immunologically tested by WHO and Malaysian specimens. The LOD of the assay was 3.0 log10 RNA copies for DENV-1, 2.0 for DENV-3, and 1.0 for DENV-2 and DENV-4. The assay showed 95.2% sensitivity (20/21) in an evaluation panel, whereas NS1 antigen- and anti-dengue IgM-based immunological assays exhibited 0% and 23.8-47.6% sensitivities, respectively. The assay showed 100% sensitivity both in NS1 antigen- and anti-dengue IgM-positive Malaysian specimens (26/26). The assay provided the information of viral loads and serotype with discrimination of heterotypic mixed infection. The assay coul...Continue Reading

References

Dec 23, 1999·The Journal of Infectious Diseases·D W VaughnA Nisalak
Feb 9, 2000·Trends in Microbiology·E C Holmes, S S Burch
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Edward C Holmes, S Susanna Twiddy
Jan 21, 2004·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·María G Guzmán, Gustavo Kourí
Aug 26, 2006·Tropical Medicine & International Health : TM & IH·M P Sánchez-SecoA Tenorio
Sep 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Wei-Kung WangChwan-Chuen King
Sep 27, 2006·Journal of Virological Methods·Yong Yean KongShamala Devi
Jul 25, 2008·Journal of Clinical Microbiology·Alexis DumoulinHans H Hirsch
Jan 27, 2009·Virology Journal·K R GurukumarD Cecilia
Feb 20, 2009·Journal of Clinical Microbiology·Jorge L Muñoz-JordánJeffrey M Linnen
Feb 26, 2009·Emerging Infectious Diseases·Elizabeth A HunspergerRosanna W Peeling
Apr 7, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Isabelle Leparc-GoffartXavier de Lamballerie
Oct 14, 2009·Clinical Microbiology Reviews·Byron E E MartinaAlbert D M E Osterhaus
Apr 21, 2010·Indian Journal of Medical Microbiology·S Datta, C Wattal
Jul 9, 2010·Clinical Microbiology Reviews·Eileen M Burd
Jul 14, 2010·PLoS Neglected Tropical Diseases·Monique da Rocha Queiroz LimaFlavia Barreto dos Santos
Oct 20, 2010·PLoS Neglected Tropical Diseases·Cristina DomingoOliver Donoso-Mantke
Nov 17, 2010·Nature Reviews. Microbiology·Maria G GuzmanRosanna W Peeling
May 7, 2011·Nature Reviews. Microbiology·Rosanna W PeelingSutee Yoksan
Jun 10, 2011·PLoS Neglected Tropical Diseases·Jenny G H LowEng Eong Ooi
May 2, 2012·Asian Pacific Journal of Tropical Medicine·Piyathida PongsiriYong Poovorawan
May 3, 2013·PLoS Neglected Tropical Diseases·Jesse J WaggonerBenjamin A Pinsky
Jul 23, 2013·PLoS Neglected Tropical Diseases·Gilberto A SantiagoJorge L Muñoz-Jordán
Dec 17, 2014·Annals of Global Health·Jin-Kyoung Oh, Elisabete Weiderpass

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Citations

Jun 29, 2016·SpringerPlus·Daniel García-RuízDarwin Elizondo-Quiroga
Dec 3, 2016·Scientific Reports·Jie YangXiancai Rao
Sep 15, 2020·Journal of Korean Medical Science·Young Jae LeeYoung Il Jeong
Nov 14, 2020·Analytical Methods : Advancing Methods and Applications·Jin ZhaoZhixu He

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