Oct 8, 2013

Clinical evaluation and biochemical analyses of thiamine deficiency in Pacific harbor seals (Phoca vitulina) maintained at a zoological facility

Journal of the American Veterinary Medical Association
Lara CroftCecilia Giulivi

Abstract

To determine thiamine-dependent enzyme activities in various tissue samples of Pacific harbor seals (Phoca vitulina) and thiaminase activities in dietary fish. Cross-sectional study. 11 Pacific harbor seals with thiamine deficiency and 5 control seals. Seals underwent evaluation to rule out various diseases and exposure to toxins. For seals that died, measurement of thiamine-dependent enzymes in liver and brain samples and determination of mitochondrial DNA (mtDNA) copy number in liver, brain, and muscle samples were performed. Thiaminase activity in dietary fish was determined. 8 seals with thiamine deficiency died. Affected seals typically had acute neurologic signs with few nonspecific findings detected by means of clinicopathologic tests and histologic examination of tissue samples. Thiamine-dependent enzyme activities in liver samples of affected seals were significantly lower than those in control liver samples. The primary activation ratios and latencies for enzymes indicated that brain tissue was more affected by thiamine deficiency than liver tissue. Activities of pyruvate dehydrogenase were more affected by thiamine deficiency than those of transketolase and ketoglutarate dehydrogenase. For control seals, the mtDNA co...Continue Reading

  • References41
  • Citations6

Citations

Mentioned in this Paper

Genus Phoca
Phocidae
Thiamine Deficiency
Diet
Thiamine Mononitrate
Enzymes, antithrombotic
TKT
DNA, Mitochondrial
Toxin
Pyruvate dehydrogenase

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