PMID: 9431924Feb 12, 1998Paper

Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples

Journal of Clinical Microbiology
G Q ZhangK Hirai

Abstract

A nested PCR method was developed for the detection of Coxiella burnetii in human serum samples. Two pairs of oligonucleotide primers were designed to amplify a 438-bp fragment of the com1 gene encoding a 27-kDa outer membrane protein of C. burnetii. The primers amplified the predicted fragments of 21 various strains of C. burnetii but did not react with DNA samples from other microorganisms. The 438-bp amplification products could be digested with restriction enzymes SspI and SalI. The utility of the nested PCR was evaluated by testing human serum samples. The com1 gene fragment was amplified from 135 (87%) of 155 indirect immunofluorescence test (IF)-positive serum samples and from 11 (11%) of 100 IF-negative serum samples. The nested PCR with primers targeted to the com1 gene appeared to be a sensitive, specific, and useful method for the detection of C. burnetii in serum samples.

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Citations

Feb 24, 2004·Microbiology and Immunology·Jung-Hee LeeSeung-Hyun Lee
Jul 27, 2005·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Akihiko HiraiSatoshi Morozumi
Feb 17, 2006·Annals of the New York Academy of Sciences·Will K ReevesGregory A Dasch
Nov 23, 2006·Annals of the New York Academy of Sciences·Noriyuki TatsumiKazuo Yamaguchi
Jan 24, 2009·Epidemiology and Infection·I KaragiannisY Van Duynhoven
May 18, 2010·Applied and Environmental Microbiology·Gilbert J KershRobert F Massung
Aug 26, 1998·The Journal of Veterinary Medical Science·K Hirai, H To
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Oct 9, 2003·The Journal of Veterinary Medical Science·Tomoyoshi KomiyaKatsuya Hirai
Jan 25, 2012·International Journal of Molecular Sciences·Ani Ioana CotarMonica Straut
Apr 6, 2013·The Journal of Veterinary Medical Science·Masako AndohShuji Ando
Jul 23, 2003·Infection and Immunity·Masako AndohKatsuya Hirai
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Oct 9, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Clare NourseJennifer M Robson
Dec 7, 2018·Transboundary and Emerging Diseases·David González-Barrio, Francisco Ruiz-Fons
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