PMID: 7512931Feb 1, 1994Paper

Clinical evaluation of a serological assay using a monoclonal antibody (TB72) to the 38 kDa antigen of Mycobacterium tuberculosis

The European Respiratory Journal
G H Bothamley, R Rudd

Abstract

We examined an enzyme-linked immunosorbent assay (ELISA) modification of a radioimmunoassay, using the TB72 monoclonal antibody, as a serological test for tuberculosis in a clinical setting. Sera were obtained from 238 patients with suspected pulmonary tuberculosis, 30 patients treated for tuberculosis, 28 contacts, and 480 random samples from inpatients. Antibody levels were measured as the dilution of serum causing 50% inhibition of binding of the TB72 monoclonal antibody, which binds to an epitope of the 38 kDa antigen specific to the Mycobacterium tuberculosis complex, a positive titre being > 3. Positive antibody titres were present in 21 out of 25 (84%) patients with smear-positive and 22 out of 27 (82%) patients with smear-negative, culture-positive tuberculosis, and 37 out of 41 (90%) patients successfully treated for tuberculosis but without bacteriological confirmation of disease. Three out of 82 (4%) patients with a firm alternative diagnosis to tuberculosis gave a positive result. Serological tests were negative within 2.5 yrs of successful treatment. Patients without a definite diagnosis one year after tuberculosis had been suspected, and those who had received inadequate treatment for tuberculosis, were frequently...Continue Reading

Citations

Jan 1, 1997·Indian Journal of Pediatrics·S Mahadevan
Jan 18, 2003·Journal of Microbiological Methods·Henriette StavriR J Doyle
Jun 19, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Elizabeth A TalbotCharles D Wells
Oct 14, 2011·Clinical and Vaccine Immunology : CVI·Walid Ben-SelmaJalel Boukadida
Jun 22, 2012·Veterinary Medicine International·Ashutosh WadhwaShigetoshi Eda
Oct 6, 1997·American Journal of Respiratory and Critical Care Medicine·I H ChiangP C Yang
May 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R DjukanovićP H Howarth
Feb 3, 2005·Scandinavian Journal of Immunology·Y Ben AmorM L Gennaro
Feb 14, 2009·Current Problems in Pediatric and Adolescent Health Care·Jennifer Lighter, Mona Rigaud
Dec 8, 2004·Diagnostic Microbiology and Infectious Disease·Alamelu RajaPatrick J Brennan
Jun 18, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S T BeckA W Ferreira
Oct 21, 2000·Journal of Clinical Pathology·C L ChangS K Park
Jun 23, 2010·Molecular BioSystems·Clara EspitiaRaúl Mancilla
Dec 5, 2008·Clinical and Vaccine Immunology : CVI·Karen R SteingartSuman Laal
Jul 7, 2000·Clinical and Diagnostic Laboratory Immunology·R L LimL Chan
Jul 3, 2002·Clinical and Diagnostic Laboratory Immunology·Raymond L HoughtonSteven G Reed
Oct 19, 2007·FEMS Immunology and Medical Microbiology·Raj Kumar Verma, Amita Jain
Jul 23, 2003·Clinical Otolaryngology and Allied Sciences·R J SalibP H Howarth
Dec 24, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Shamsi YariAhmadreza Bahrmand
Dec 10, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David M LewinsohnGail L Woods
Apr 1, 1996·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·E O BasseyC Raykundalia
Aug 1, 1996·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R A ColeA T Zhou

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