Clinical, laboratory, and temporal predictors of neutralizing antibodies to SARS-CoV-2 after COVID-19.

MedRxiv : the Preprint Server for Health Sciences
Jim BoonyaratanakornkitDavid M Koelle

Abstract

SARS-CoV-2-specific antibodies may protect from reinfection and disease, providing the rationale for administration of plasma containing SARS-CoV-2 neutralizing antibodies (nAb) as a treatment for COVID-19. The clinical factors and laboratory assays to streamline plasma donor selection, and the durability of nAb responses, are incompletely understood. Adults with virologically-documented SARS-CoV-2 infection in a convalescent plasma donor screening program were tested for serum IgG to SARS-CoV-2 spike protein S1 domain, nucleoprotein (NP), and for nAb. Amongst 250 consecutive persons studied a median of 67 days since symptom onset, 243/250 (97%) were seropositive on one or more assays. Sixty percent of donors had nAb titers ≥1:80. Correlates of higher nAb titer included older age (adjusted OR [AOR] 1.03/year of age, 95% CI 1.00-1.06), male sex (AOR 2.08, 95% CI 1.13-3.82), fever during acute illness (AOR 2.73, 95% CI 1.25-5.97), and disease severity represented by hospitalization (AOR 6.59, 95% CI 1.32-32.96). Receiver operating characteristic (ROC) analyses of anti-S1 and anti-NP antibody results yielded cutoffs that corresponded well with nAb titers, with the anti-S1 assay being slightly more predictive. NAb titers declined i...Continue Reading

Citations

Feb 16, 2021·Frontiers in Immunology·Stephan SchlickeiserLeif G Hanitsch
Apr 8, 2021·JMIR Public Health and Surveillance·Henry T PengAndrew Beckett

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Datasets Mentioned

BETA
MN908947.3

Methods Mentioned

BETA
PCR
blood draw
blood draws
density gradient centrifugation
ELISA

Clinical Trials Mentioned

NCT04344977
NCT04338360

Software Mentioned

SPSS
Operetta
R package lme4

Related Concepts

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