Clinical outcome of HIV viraemic controllers and noncontrollers with normal CD4 counts is exclusively determined by antigen-specific CD8+ T-cell-mediated HIV suppression

PloS One
Yada TansiriPokrath Hansasuta

Abstract

In this cross-sectional study we evaluated T-cell responses using several assays to determine immune correlates of HIV control that distinguish untreated viraemic controllers (VC) from noncontrollers (NC) with similar CD4 counts. Samples were taken from 65 ART-naïve chronically HIV-infected VC and NC from Thailand with matching CD4 counts in the normal range (>450 cells/μl). We determined HIVp24-specific T-cell responses using standard Interferon-gamma (IFNγ) ELISpot assays, and compared the functional quality of HIVp24-specific CD8+ T-cell responses using polychromatic flow cytometry. Finally, in vitro HIV suppression assays were performed to evaluate directly the activity of CD8+ T cells in HIV control. Autologous CD4+ T cells were infected with primary patient-derived HIV isolates and the HIV suppressive activity of CD8+ T cells was determined after co-culture, measuring production of HIVp24 Ag by ELISA. The HIVp24-specific T-cell responses of VC and NC could not completely be differentiated through measurement of IFNγ-producing cells using ELISpot assays, nor by the absolute cell numbers of polyfunctional HIVp24-specific CD8+ T cells. However, in vitro HIV suppression assays showed clear differences between VC and NC. HIV s...Continue Reading

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Citations

Mar 28, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bo XiangAdam E Snook
Aug 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mathieu AnginUNKNOWN ANRS CO5 IMMUNOVIR-2 Study Group
Jun 3, 2017·BMC Infectious Diseases·Samara Tatielle M GomesRicardo Ishak

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Methods Mentioned

BETA
flow cytometry
density gradient centrifugation
FACS
ELISA

Software Mentioned

FlowJo
GraphPad Prism

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