Clinical Performance of a Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Method for Detection of Certain blaKPC-Containing Plasmids

Journal of Clinical Microbiology
Jung-Ho YounAnna F Lau

Abstract

Rapid detection of blaKPC-containing organisms can significantly impact infection control and clinical practices, as well as therapeutic choices. Current molecular and phenotypic methods to detect these organisms, however, require additional testing beyond routine organism identification. In this study, we evaluated the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to detect pKpQIL_p019 (p019)-an ∼11,109-Da protein associated with certain blaKPC-containing plasmids that was previously shown to successfully track a clonal outbreak of blaKPC-pKpQIL-Klebsiella pneumoniae in a proof-of-principle study (A. F. Lau, H. Wang, R. A. Weingarten, S. K. Drake, A. F. Suffredini, M. K. Garfield, Y. Chen, M. Gucek, J. H. Youn, F. Stock, H. Tso, J. DeLeo, J. J. Cimino, K. M. Frank, and J. P. Dekker, J Clin Microbiol 52:2804-2812, 2014, http://dx.doi.org/10.1128/JCM.00694-14). PCR for the p019 gene was used as the reference method. Here, blind analysis of 140 characterized Enterobacteriaceae isolates using two protein extraction methods (plate extraction and tube extraction) and two peak detection methods (manual and automated) showed sensitivities and specificities ranging f...Continue Reading

References

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Citations

Apr 8, 2016·BMC Microbiology·Ian A MylesSandip K Datta
Apr 5, 2016·Expert Review of Molecular Diagnostics·Yasufumi Matsumura, Johann D Pitout
Aug 2, 2016·JCI Insight·Ian A MylesSandip K Datta
Jul 8, 2017·British Journal of Pharmacology·Wei ZhengAnton Simeonov
Sep 1, 2018·Journal of Medical Microbiology·Anna Rita CentonzeAnnarita Mazzariol
Feb 26, 2016·Journal of Clinical Microbiology·Thomas J GniadekPatricia J Simner
Aug 31, 2018·Journal of Clinical Microbiology·Pranita D Tamma, Patricia J Simner
Jun 18, 2016·Proteomics. Clinical Applications·Yannick Charretier, Jacques Schrenzel
Jun 30, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ioannis K Neonakis, Demetrios A Spandidos
Jan 25, 2017·Future Science OA·Andrew M RobinsonJames E Ussher
Jun 2, 2017·Scientific Reports·Honghui WangJohn P Dekker
Jan 29, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Otávio A LovisonAndreza F Martins
Dec 14, 2018·Frontiers in Microbiology·Miriam CordovanaArthur B Pranada
Nov 30, 2021·Journal of Microbiological Methods·Yun HuangCongrong Li

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