Clinical Proteomics and Cytokine Profiling for Dengue Fever Disease Severity Biomarkers

Omics : a Journal of Integrative Biology
Manali JadhavSanjeeva Srivastava

Abstract

Dengue fever (DF) is a major global health burden with a pathophysiology that is still incompletely understood. Biomarkers that predict and explain susceptibility to DF and its progression to its more severe hemorrhagic form are much needed. DF is endemic in tropical and subtropical regions of the world, with a rapidly increasing incidence of disease severity. We conducted a clinical biomarker discovery study using both a case-control and longitudinal study design. Plasma proteome alterations in patients with DF (n = 12) and dengue hemorrhagic fever (DHF, n = 24) were analyzed in comparison to healthy controls (HCs, n = 16), using the isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomics methodology (false discovery rate of 1%, ≥2 peptides). Several proteins such as the alpha-2 macroglobulin, angiotensinogen, apolipoprotein B-100, serotransferrin, and ceruloplasmin were upregulated (fold change >1.2) in all DHF cases, and downregulated in DF (fold change <0.83), compared with HCs. Plasma cytokine profiling (8 DF, 8 DHF, and 8 HC) on two consecutive time points, at day 0 (day of admission) and days 5-7, found significant elevation in IL-1RA, IL-7, TNF-α, MCP1-MCAF, and MIP-1β levels, but on...Continue Reading

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Citations

Mar 19, 2019·Omics : a Journal of Integrative Biology·Yi-Ning DaiHai-Jun Huang
Jun 25, 2020·Expert Review of Proteomics·Khushman TaunkSrikanth Rapole
Jun 13, 2020·Frontiers in Cellular and Infection Microbiology·Priyank JaiswalSumi Mukhopadhyay
Oct 27, 2021·Virulence·Sophie Wilhelmina van LeurSumana Sanyal
Jan 23, 2022·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Mojgan MasoodiThomas Renné

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Methods Mentioned

BETA
electrophoresis
GTPase
Protein ANalysis

Software Mentioned

DAVID
Plex Manager
PANTHER
Bio
Spectrum Mill
ImageQuant
GraphPad Prism
DAVID ( Database for Annotation , Visualization , and Integrat...
PANTHER ( Protein ANalysis THrough Evolutionary Relationships )

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