PMID: 3772309Sep 1, 1986Paper

Clinical relevance of alkaline phosphatase isoenzyme determinations by high performance liquid chromatography

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
E SchönauH J Böhles

Abstract

A new method for the separation of alkaline phosphatase isoenzymes by means of high performance liquid chromatography (HPLC) is presented. One isoenzyme was identified in homogenate of small intestine, two were identified in bone, and two in liver, and fragment and biliary isoenzymes were identified in bile. Sera from 32 patients with different diseases of the skeletal system or the liver were analysed. High activities of the bone isoenzymes were detected in bone diseases, of the second liver isoenzyme in acute hepatitis and of the first liver and biliary isoenzymes in biliary obstruction. There are indications that the first liver isoenzyme is derived from the cell membrane and the second liver isoenzyme from the cytosol. The biliary isoenzyme is considered to be a highly sensitive and specific indicator for cholestasis.

References

Nov 2, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·P M Crofton, A F Smith
Aug 1, 1971·Clinica Chimica Acta; International Journal of Clinical Chemistry·A W Walker, A C Pollard
Mar 14, 1969·Deutsche medizinische Wochenschrift·U E Klein
Sep 1, 1967·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Bahr, J H Wilkinson
Mar 1, 1982·Critical Reviews in Clinical Laboratory Sciences·P M Crofton
Mar 5, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A Stinson, L E Seargeant

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Citations

Feb 28, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·M MiuraT Komoda
Jan 15, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·T KomodaT Sekine
Apr 1, 1989·Clinical Biochemistry·U B SeiffertG Oremek
Dec 1, 1988·European Journal of Pediatrics·E SchönauH J Böhles

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