Clinical relevance of ErbB-2/HER2 nuclear expression in breast cancer.

BMC Cancer
Roxana SchillaciPatricia V Elizalde

Abstract

The biological relevance of nuclear ErbB-2/HER2 (NuclErbB-2) presence in breast tumors remains unexplored. In this study we assessed the clinical significance of ErbB-2 nuclear localization in primary invasive breast cancer. The reporting recommendations for tumor marker prognostic studies (REMARK) guidelines were used as reference. Tissue microarrays from a cohort of 273 primary invasive breast carcinomas from women living in Chile, a Latin American country, were examined for membrane (MembErbB-2) and NuclErbB-2 expression by an immunofluorescence (IF) protocol we developed. ErbB-2 expression was also evaluated by immunohistochemistry (IHC) with a series of antibodies. Correlation between NuclErbB-2 and MembErbB-2, and between NuclErbB-2 and clinicopathological characteristics of tumors was studied. The prognostic value of NuclErbB-2 in overall survival (OS) was evaluated using Kaplan-Meier method, and Cox model was used to explore NuclErbB-2 as independent prognostic factor for OS. The IF protocol we developed showed significantly higher sensitivity for detection of NuclErbB-2 than IHC procedures, while its specificity and sensitivity to detect MembErbB-2 were comparable to those of IHC procedures. We found 33.6% NuclErbB-2 p...Continue Reading

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Citations

Dec 30, 2014·Journal of Mammary Gland Biology and Neoplasia·A DittrichA Tyson-Capper
Oct 22, 2016·Endocrine-related Cancer·Patricia V ElizaldeRoxana Schillaci
Dec 4, 2016·Cancer Metastasis Reviews·Jennifer L Hsu, Mien-Chie Hung
Jan 19, 2019·Hormones & Cancer·Rosalía I Cordo RussoPatricia V Elizalde
Jul 7, 2020·Journal of Cellular Biochemistry·Guoqiang HuaJean Imbert
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Jul 8, 2020·Hormones & Cancer·Santiago MaderaPatricia V Elizalde
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Dec 12, 2020·Journal of Experimental & Clinical Cancer Research : CR·Giulia BonPatrizia Vici
Jun 18, 2021·Journal of Cancer Research and Clinical Oncology·Vic HartAlison Tyson-Capper

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