Clinical Remission of Sight-Threatening Non-Infectious Uveitis Is Characterized by an Upregulation of Peripheral T-Regulatory Cell Polarized Towards T-bet and TIGIT

Frontiers in Immunology
Rose M GilbertSue Lightman

Abstract

Non-infectious uveitis can cause chronic relapsing and remitting ocular inflammation, which may require high dose systemic immunosuppression to prevent severe sight loss. It has been classically described as an autoimmune disease, mediated by pro-inflammatory Th1 and Th17 T-cell subsets. Studies suggest that natural immunosuppressive CD4+CD25+FoxP3+ T-regulatory cells (Tregs) are involved in resolution of inflammation and may be involved in the maintenance of clinical remission. To investigate whether there is a peripheral blood immunoregulatory phenotype associated with clinical remission of sight-threatening non-infectious uveitis by comparing peripheral blood levels of Treg, Th1, and Th17, and associated DNA methylation and cytokine levels in patients with active uveitic disease, control subjects and patients (with previously active disease) in clinical remission induced by immunosuppressive drugs. Isolated peripheral blood mononuclear cells (PBMC) from peripheral blood samples from prospectively recruited subjects were analyzed by flow cytometry for CD3, CD4, FoxP3, TIGIT, T-bet, and related orphan receptor γt. Epigenetic DNA methylation levels of FOXP3 Treg-specific demethylated region (TSDR), FOXP3 promoter, TBX21, RORC2,...Continue Reading

References

Sep 1, 1996·The British Journal of Ophthalmology·M S Suttorp-Schulten, A Rothova
Jan 11, 2003·Science·Shohei HoriShimon Sakaguchi
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Oct 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Bruce Richardson
Jan 9, 2004·Advances in Immunology·Shohei HoriShimon Sakaguchi
Mar 24, 2004·Seminars in Immunology·David A HorwitzSatoshi Yamagiwa
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Song Guo ZhengDavid A Horwitz
Jul 29, 2004·The Journal of Experimental Medicine·Michael R EhrensteinClaudia Mauri
Aug 20, 2004·The British Journal of Ophthalmology·O M DurraniP I Murray
Mar 5, 2005·Ophthalmic Research·Necip TorunUwe Pleyer
Oct 1, 2005·American Journal of Ophthalmology·Douglas A JabsUNKNOWN Standardization of Uveitis Nomenclature (SUN) Working Group
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Kenneth G-J OoiVirginia L Calder
Mar 28, 2006·Investigative Ophthalmology & Visual Science·Hiroshi TakaseManabu Mochizuki
Apr 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marc A GavinAlexander Y Rudensky
Aug 19, 2006·The British Journal of Ophthalmology·G J WilliamsJ A Olson
Dec 13, 2006·European Journal of Immunology·Jun WangRené E M Toes
Jan 11, 2007·Clinical Medicine & Research·Kenneth G-J OoiSusan L Lightman
Feb 15, 2007·PLoS Biology·Stefan FloessJochen Huehn
Sep 14, 2007·Current Eye Research·M KramerN Goldenberg-Cohen
Feb 21, 2008·PloS One·Peter C J JansonOla Winqvist
May 20, 2008·Investigative Ophthalmology & Visual Science·Yan KeHui Shao
May 22, 2008·European Journal of Immunology·Julia K PolanskyJochen Huehn
May 28, 2008·The Journal of Experimental Medicine·Matthias LochnerGérard Eberl
Dec 31, 2008·Nature Reviews. Immunology·Jochen HuehnAlf Hamann
Feb 25, 2009·Immunopharmacology and Immunotoxicology·Lauren P SchewitzAndrew D Dick
Apr 3, 2009·Investigative Ophthalmology & Visual Science·Richard W J LeeAndrew D Dick
Apr 15, 2009·Archives of Ophthalmology·Steven YehRobert B Nussenblatt
Aug 22, 2009·Investigative Ophthalmology & Visual Science·Min SunAize Kijlstra
May 21, 2010·European Journal of Immunology·Laura MaggiFrancesco Annunziato
Jul 21, 2010·Nature Immunology·Kenneth M Murphy, Brigitta Stockinger
Jan 5, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole JollerVijay K Kuchroo
Apr 1, 2011·Investigative Ophthalmology & Visual Science·Rachel R Caspi
Apr 16, 2011·Bioinformatics·Felix Krueger, Simon R Andrews
Aug 11, 2011·FEBS Letters·Konstantia-Maria Chavele, Michael R Ehrenstein
May 9, 2012·Ocular Immunology and Inflammation·Simona RuggieriSilvana Guerriero
Jan 18, 2013·Digestive Diseases·Jean-Frédéric ColombelRemo Panaccione
Jan 29, 2013·Current Gastroenterology Reports·Camille Zallot, Laurent Peyrin-Biroulet

❮ Previous
Next ❯

Citations

Oct 26, 2018·Frontiers in Immunology·Igal Gery, Rachel R Caspi
Jun 19, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xuewei YinDadong Guo
Oct 9, 2019·Seminars in Immunopathology·Gerhild Wildner, Maria Diedrichs-Möhring
Jan 26, 2021·Frontiers in Immunology·Roxane L Degroote, Cornelia A Deeg
Apr 4, 2021·International Journal of Molecular Sciences·Dan-In JangSeung-Hoon Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
ELISA
PMA
Amplicon
Illumina sequencing
Assay
PCR
Amplicon Sequencing
flow

Software Mentioned

Bismark
GraphPad Prism
FlowJo
Fluidigm
SPSS
BD FACSDiva
Pyromark Assay Design
Modfit LT
Modfit

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Stephanie A McClymontTodd M Brusko
Ocular Immunology and Inflammation
Ravi K SharmaNaresh Sachdeva
Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology
Monique R AndersonSteven Jacobson
© 2022 Meta ULC. All rights reserved