PMID: 15373223Sep 18, 2004Paper

Clinical results of tissue-engineered vascular autografts seeded with autologous bone marrow cells

Nihon Geka Gakkai zasshi
Toshiharu Shin'oka

Abstract

In May 2000 we initiated clinical application of tissue-engineered (TE) vascular grafts seeded with cultured cells. However, cell culturing is time-consuming and xeno-serum must be used. To overcome these disadvantages, we started to use bone marrow cells (BMCs), readily available on the day of surgery, as a cell source. The aim of this study was to assess the safety and feasibility of this technique for creating pulmonary arteries. Since August 2000, TE grafts seeded with autologous BMCs have been implanted in 42 patients. Bone marrow (5 ml/kg) was aspirated under general anesthesia prior to the skin incision. The polymer tube serving as a scaffold for the cells was composed of a co-polymer of 1-lactide and epsilon-caprolactone (50:50). This co-polymer is degraded by hydrolysis. The matrix is >80% porous and the diameter of each pore is 100-200 microm. Polyglycolic acid woven fabric with a thickness of 0.5 mm was used for reinforcement. Twenty-three TE conduits (TCPC grafts) and 19 TE patches were used for the repair of congenital heart defects. The patients' ages ranged from 1 to 24 years (median, 5.5 years). All patients underwent a catheterization study and/or computed tomography (CT) scans for evaluation after surgery. The...Continue Reading

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