Clinical Significance and Tumor-Suppressive Function of miR-516b in Nonsmall Cell Lung Cancer

Cancer Biotherapy & Radiopharmaceuticals
Junfei ZhuLitong Jin

Abstract

MicroRNA-516b (miR-516b) has been recently reported to be downregulated in nonsmall cell lung cancer (NSCLC). However, its clinical significance and biological function in NSCLC remain to be clarified. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-516b in 82 paired fresh primary tumor tissues and NSCLC cell lines. The association of miR-516b expression with clinicopathological factors and prognosis was statistically analyzed by SPSS 21.0 software, Kaplan-Meier method, and Cox regression analyses. Cell Counting Kit-8, colony formation, flow cytometric, Transwell migration, and invasion assays were used to evaluate the proliferation, cell cycle, apoptosis, migration, and invasion of NSCLC cells after miR-516b mimics or negative control of mimics transfection. The expression level of miR-516b was found to be significantly lower in NSCLC tissues and cell lines than in corresponding normal tissues and cells. Decreased miR-516b expression was significantly associated with tumor size (p = 0.004), Tumor Node Metastasis (TNM) stage (p = 0.016), and shorter overall survival (p = 0.0039). Multivariate analysis suggested that miR-516b was an independent risk factor for NSCLC (hazard rat...Continue Reading

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Methods Mentioned

BETA
protein assay
transfection
flow cytometry

Software Mentioned

SPSS

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