PMID: 7011327Apr 1, 1981Paper

Clinical significance of anti-double-stranded DNA antibodies detected by a solid phase enzyme immunoassay

Arthritis and Rheumatism
T E MillerD Koffler

Abstract

A solid phase enzyme immunoassay (EIA) detected anti-double-stranded (ds) DNA antibodies in 88% of sera from patients classified clinically as having active systemic lupus erythematosus (SLE) without renal symptoms and 93% with renal disease. Fifty-six percent of sera from patients with inactive SLE were EIA positive for anti-dsDNA antibodies. The EIA had a sensitivity and specificity comparable to radioimmunoassay (RIA) and hemagglutination for patients with active SLE with or without renal disease, but it detected anti-dsDNA antibodies more frequently in patients with inactive SLE than the latter procedures. Precipitating antibodies detected by counterimmunoelectrophoresis (CIE) were less common in patients with renal disease (23% incidence) than clinically active patients without renal disease (79% incidence). Twenty-four SLE sera with elevated levels of C1q binding showed a 96% concordance for a positive EIA for anti-dsDNA antibodies in contrast to 66% concordance by RIA or hemagglutination. These findings suggest that the EIA is a sensitive and specific method for detection and measurement of anti-dsDNA antibodies. Several clinical applications of the EIA are discussed.

References

Jan 1, 1977·Arthritis and Rheumatism·S A LeonB Shapiro
Aug 1, 1978·Clinical Immunology and Immunopathology·R D Sontheimer, J N Gilliam
Mar 1, 1979·Arthritis and Rheumatism·R L RileyR P Taylor
Jan 1, 1979·Journal of Immunological Methods·S AotsukaR Yokohari
Dec 1, 1977·The Journal of Clinical Investigation·J B PennebakerM Ziff
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G H Kelsoe, T H Weller
Jan 1, 1979·Journal of Immunological Methods·J L KlotzR L Teplitz
Jan 1, 1976·Journal of Immunological Methods·L A ArdenT E Feltkamp
Jan 1, 1974·Annual Review of Medicine·D Koffler
Nov 1, 1974·Arthritis and Rheumatism·M E Gershwin, A D Steinberg
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Mar 7, 1968·The New England Journal of Medicine·P H Schur, J Sandson
Jul 1, 1971·The Journal of Experimental Medicine·D KofflerH G Kunkel
Dec 26, 1969·Science·D KofflerH G Kunkel
Nov 1, 1967·The Journal of Clinical Investigation·R Arana, M Seligmann
Jan 1, 1959·The Journal of Experimental Medicine·H R DEICHERH G KUNKEL

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Citations

Oct 17, 2002·Arthritis and Rheumatism·Arthur F KavanaughUNKNOWN American College of Rheumatology Ad Hoc Committee on Immunologic Testing Guidelines
Apr 13, 2013·Immunologic Research·David S Pisetsky
Nov 28, 1985·Journal of Immunological Methods·K Yoshikawa, M Fukada
Aug 28, 1990·Journal of Immunological Methods·W EmlenG Burdick
Apr 8, 1992·Journal of Immunological Methods·G KroubouzosA Varelzidis
Oct 28, 1983·Journal of Immunological Methods·R L RubinE M Tan
Feb 4, 1982·The New England Journal of Medicine·G BieseckerD Koffler
Dec 1, 1981·The Journal of Experimental Medicine·G BieseckerD Koffler
Sep 1, 1994·The Journal of Experimental Medicine·J B KatzB Diamond
Jun 1, 1987·Annals of the Rheumatic Diseases·D A IsenbergA Todd-Pokropek
Dec 1, 1986·Arthritis and Rheumatism·W EmlenR P Taylor
Jan 1, 1990·Journal of Clinical Laboratory Analysis·J L Preud'homme
Nov 23, 2019·International Journal of Molecular Sciences·Gian Marco GhiggeriMaurizio Bruschi
May 1, 1983·Human Pathology·D KofflerI Faiferman

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