Clinical surveillance for human astrovirus in Monastir region, Tunisia

BMC Public Health
Abir MonastiriJordi Serra-Cobo

Abstract

Astroviruses (AstVs) are enteric viruses that can cause gastroenteritis in children. This study is part of monitoring the circulation of astroviruses in children hospitalized and/or outpatients for acute gastroenteritis at the primary care center of Ouerdanine or at the Pediatric Department of the University Hospital Fattouma-Bourguiba (Monastir, Tunisia). The aims of our study were to know the prevalence of human astrovirus in clinical samples of children, characterize the strains and evaluate the infectivity of isolated strains on cell culture. Fifty stool samples were collected from children under five years old in the region of Monastir (Tunisia) from October 2010 to June 2011. All specimens were subjected to RT-PCR amplification followed by sequencing and phylogenetic analysis. The study shows a low prevalence of astrovirus (4 %) in children. The two positive samples obtained were HAstV type 3. Samples that were RT-PCR positive were cultured in CaCO-2 cells and the presence of infectious viral particles was confirmed. The phylogenetic analysis shows that the different HAstV-3 strains isolated in Tunisia are grouped into two clusters. The first cluster includes strains obtained in 2004, which belong to lineage HAstV-3a, whi...Continue Reading

References

Oct 24, 1998·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·H MustafaR F Bishop
Aug 19, 2000·The Journal of Infectious Diseases·A B NaficyJ D Clemens
Nov 22, 2000·The Journal of General Virology·M Méndez-TossC F Arias
Jul 11, 2001·Novartis Foundation Symposium·S M MatsuiU Geigenmüller-Gnirke
Jan 5, 2002·Journal of Clinical Microbiology·Susana GuixRosa M Pintó
Jun 25, 2003·Current Opinion in Infectious Diseases·Jolan E Walter, Douglas K Mitchell
Nov 20, 2003·FEMS Immunology and Medical Microbiology·Ferenc JakabGyörgy Szucs
Feb 22, 2008·Journal of Clinical Microbiology·Khira Sdiri-LouliziPierre Pothier
Aug 28, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Madhusudhan PativadaTriveni Krishnan
May 3, 2013·PloS One·Muhammad Masroor AlamSyed Sohail Zahoor Zaidi
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 5, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mokibul Hassan AfradTasnim Azim
Oct 4, 2014·Clinical Microbiology Reviews·Albert BoschSusana Guix
Mar 19, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Maria Cristina MediciAdriana Calderaro

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Citations

Jul 3, 2021·Viruses·Ignacio Parrón On Behalf Of The Working Group For The Study Of Acute Gastroenteritis Outbreaks In Catalonia

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Datasets Mentioned

BETA
KC896091
AB551378
JQ045383

Methods Mentioned

BETA
PCR

Software Mentioned

Mega
Sequencher

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