Clinical utility of fractionating erythrocytes into "Percoll" density gradients

Advances in Experimental Medicine and Biology
A MoscaR Galanello

Abstract

Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.

Citations

May 28, 2020·Frontiers in Physiology·Anna BogdanovaAsya Makhro

Related Concepts

Percoll
Microangiopathic Hemolytic Anemia
Cell Fractionation
Centrifugation, Density Gradient
Microencapsulation
Clinical Enzyme Tests (Procedure)
Erythrocyte Survival
Erythrocytes
Aldolase C
Glucosephosphate Dehydrogenase

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