Clinical utility of fractionating erythrocytes into "Percoll" density gradients

Advances in Experimental Medicine and Biology
A MoscaR Galanello


Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.


May 28, 2020·Frontiers in Physiology·Anna BogdanovaAsya Makhro

Related Concepts

Microangiopathic Hemolytic Anemia
Cell Fractionation
Centrifugation, Density Gradient
Clinical Enzyme Tests (Procedure)
Erythrocyte Survival
Aldolase C
Glucosephosphate Dehydrogenase

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