Clinical validation of a point-of-care multiplexed in vitro immunoassay using monoclonal antibodies (the MSD influenza test) in four hospitals in Vietnam.

Journal of Clinical Microbiology
H Rogier van DoornMenno D de Jong

Abstract

Point-of-care (POC) diagnostic tests for influenza can considerably shorten the time to clinical decision making. An investigational POC test based on a multiplexed immunoassay was developed by Meso Scale Diagnostics, LLC (MSD), with the objective to make a more sensitive rapid test that can also subtype influenza A viruses (1977 H1, H3, and H5). Between February and November 2010, we conducted a prospective multicenter study at four hospitals in Vietnam and compared the performance of this test to that of the WHO/CDC real-time reverse transcriptase PCR (RT-PCR) on nasal and throat swab specimens from patients presenting with influenza-like illness. Five hundred sixty-three adults and children with a median age of 25 months were enrolled. Sensitivity and specificity of the test with combined results from nasal and throat swab samples were 74.0% (131/177) and 99.7% (351/352), respectively, compared to RT-PCR. The POC test was as sensitive for influenza virus B as for influenza virus A (74.4% [64/86] versus 73.6% [67/91]). The positivity rate was associated with lower cycle threshold values (a marker for higher viral loads), sample type (73.6% for nasal swab versus 52.4% for throat swab), and younger age. A total of 210 (18.7%) o...Continue Reading

References

Feb 27, 2004·The New England Journal of Medicine·Tinh Hien TranUNKNOWN World Health Organization International Avian Influenza Investigative Team
Apr 20, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T WeitzelB Schweiger
Apr 25, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Aeron C HurtIan G Barr
Dec 18, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Andrea T CruzGail J Demmler
Jul 22, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Marie L LandryDavid Ferguson
Feb 3, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C SteiningerT Popow-Kraupp
Mar 28, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Timothy M UyekiArnold S Monto
Jul 10, 2009·Journal of Clinical Microbiology·Isabelle RouleauGaston De Serres
Dec 24, 2009·Emergency Medicine Journal : EMJ·C BiggsK T Iacono
Mar 5, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pauline M LucasVictor H MacIntosh
Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Steven M BabinCharlotte A Gaydos

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Citations

Oct 19, 2017·Diagnostics·Chiuan Herng LeowJames McCarthy
Oct 17, 2014·Journal of Clinical Microbiology·Adriana PeciJonathan B Gubbay
Jul 6, 2014·Journal of Clinical Microbiology·Shuping NieN Esther Babady

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