Clinically applicable magnetic-labeling of natural killer cells for MRI of transcatheter delivery to liver tumors: preclinical validation for clinical translation

Kangan LiZhuoli Zhang


To test the hypothesis that MRI can monitor intraportal vein (IPV) transcatheter delivery of clinically applicable heparin-protamine-ferumoxytol (HPF) nanocomplex-labeled natural killer (NK) cells to liver tumor. Liver tumor rat models underwent catheterization for IPV infusion of HPF-labeled NK cells (NK-92MI cell line). MRI measurements within tumor and adjacent liver tissues were compared pre- and post-NK cell infusion. Histology studies were used to identify NK cells in the target tumors. For first time, we demonstrated that MRI tracks HPF-labeled NK cells migration within liver following IPV delivery. IPV transcatheter infusion permitted selective delivery of NK cells to liver tissues and MRI allowed tracking NK cell biodistributions within the tumors.


Jan 1, 1989·Advances in Immunology·G Trinchieri
Aug 28, 2001·Journal of Hematotherapy & Stem Cell Research·T TonnE Seifried
Mar 22, 2002·Nature Reviews. Immunology·A Cerwenka, L L Lanier
Aug 13, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jeff W M BulteJoseph A Frank
Dec 20, 2003·European Journal of Nuclear Medicine and Molecular Imaging·B MellerManfred Baehre
Nov 5, 2004·NMR in Biomedicine·Jeff W M Bulte, Dara L Kraitchman
Aug 18, 2005·Immunologic Research·Wayne M Yokoyama
Nov 16, 2006·Journal of Translational Medicine·Lina MateraTheresa L Whiteside
Dec 22, 2006·PloS One·Karen S AboodyMary K Danks
Apr 18, 2007·Nature Reviews. Immunology·Hans-Gustaf Ljunggren, Karl-Johan Malmberg
Sep 28, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jong-Kai HsiaoHon-Man Liu
May 29, 2008·European Radiology·Elizabeth J SuttonHeike Daldrup-Link
Jul 1, 2008·Nuclear Medicine and Biology·Reinhard MeierHeike Daldrup-Link
Dec 18, 2008·Cytotherapy·S SrivastavaR W Childs
Jul 21, 2009·Journal of Internal Medicine·Tolga Sutlu, Evren Alici
Aug 12, 2009·Contrast Media & Molecular Imaging·Tobias D HenningHeike Daldrup-Link
Jul 16, 2010·Cancer Research·Priyanka JhaHeike Daldrup-Link
Oct 12, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Reinhard MeierHeike Daldrup-Link
Nov 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lu-En WaiSheri M Krams
Jun 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maciej KmieciakMasoud H Manjili
Apr 6, 2012·Japanese Journal of Radiology·Shiro MiyayamaOsamu Matsui
Sep 15, 2012·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Kenneth R MeehanMarc S Ernstoff
Jan 11, 2013·Cancer Research·Ryan AlkinsKullervo Hynynen
Apr 23, 2013·Cellular & Molecular Immunology·Min ChengZhigang Tian
Jul 6, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wing Leung

Methods Mentioned

x-ray angiography
fluorescence-activated cell sorting

Clinical Trials Mentioned


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