Clinically relevant concentration determination of inhaled anesthetics (halothane, isoflurane, sevoflurane, and desflurane) by 19F NMR

Cell Biochemistry and Biophysics
Pravat K Mandal, Jay W Pettegrew

Abstract

Biophysical studies of protein-anesthetic interactions using nuclear magnetic resonance (NMR) spectroscopy are often conducted by the addition of micro amounts of neat inhaled anesthetic which yields much higher than clinically relevant (0.2-0.5 mM) anesthetic concentrations. We report a 19F NMR technique to measure clinically relevant inhaled anesthetic concentrations from saturated aqueous solutions of these anesthetics (halothane, isoflurane, sevoflurane, and desflurane). We use a setup with a 3-mm NMR tube (containing trifluoroacetic acid as standard), coaxially inserted in a 5-mm NMR tube containing anesthetic solution under investigation. All experiments are conducted in a 5-mm NMR probe. We also have provided standard curves for four inhaled anesthetics using NMR technique. The standard curve for each of these anesthetics is helpful in determining the prerequisite amount of aqueous anesthetic solution required to prepare clinically relevant concentrations for protein-anesthetic interaction studies.

References

Jan 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·T SetoY Taniguchi
Jul 1, 1993·British Journal of Anaesthesia·N P Franks, W R Lieb
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·B W DuboisA S Evers
Jul 11, 2003·The Journal of Biological Chemistry·Ming FangRoger A Johns
Jun 4, 2004·The Journal of Biological Chemistry·Sergej MilovicLukas G Weigl
Jul 24, 2004·Anesthesia and Analgesia·Akira KitamuraToshio Narahashi

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Citations

Oct 11, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Maria I LioudynoMichael T Alkire
Jan 1, 2009·Biochemical and Biophysical Research Communications·Pravat K Mandal, Vincenzo Fodale
May 20, 2009·European Journal of Anaesthesiology·Pravat K Mandal, Vincenzo Fodale
Oct 4, 2019·Current Medicinal Chemistry·Yuzhong WangCheng-Pan Zhang

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