Clinically relevant inflammatory breast cancer patient-derived xenograft-derived ex vivo model for evaluation of tumor-specific therapies

PloS One
Bedrich L EckhardtGeoffrey A Bartholomeusz

Abstract

Inflammatory breast cancer (IBC) is a rare and aggressive presentation of invasive breast cancer with a 62% to 68% 5-year survival rate. It is the most lethal form of breast cancer, and early recognition and treatment is important for patient survival. Like non-inflammatory breast cancer, IBC comprises multiple subtypes, with the triple-negative subtype being overrepresented. Although the current multimodality treatment regime of anthracycline- and taxane-based neoadjuvant therapy, surgery, and radiotherapy has improved the outcome of patients with triple-negative IBC, overall survival continues to be worse than in patients with non-inflammatory locally advanced breast cancer. Translation of new therapies into the clinics to successfully treat IBC has been poor, in part because of the lack of in vitro preclinical models that can accurately predict the response of the original tumor to therapy. We report the generation of a preclinical IBC patient-derived xenograft (PDX)-derived ex vivo (PDXEx) model and show that it closely replicates the tissue architecture of the original PDX tumor harvested from mice. The gene expression profile of our IBC PDXEx model had a high degree of correlation to that of the original tumor. This sugge...Continue Reading

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Citations

May 11, 2019·PloS One·Michael E BregenzerGeeta Mehta
Jul 22, 2021·ACS Biomaterials Science & Engineering·Gowri Manohari BalachanderKaushik Chatterjee

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Datasets Mentioned

BETA
GTX16667

Methods Mentioned

BETA
xenograft
in vitro transcription
xenografts
biopsy

Software Mentioned

PDXEx
R
Transcriptome Analysis Console

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