PMID: 9451427Feb 6, 1998Paper

Clinico-prognostic relevance of quantitative immunophenotyping in B-cell chronic lymphocytic leukemia with emphasis on the expression of CD20 antigen and surface immunoglobulins

European Journal of Haematology
Stefano MolicaA Mannella

Abstract

Expression of CD20, evaluated as antibody binding capacity (ABC) (i.e. absolute number of molecules of antibody per cell), was analyzed using flow cytometry on leukemic cells of 93 previously untreated patients, all fulfilling strict criteria of "immunologically typical" (i.e. CD5+, CD23+) B-cell chronic lymphocytic leukemia (CLL). Although changes of CD20 antigen density did not correlate with clinical parameters representative of either tumor mass (i.e. clinical stage, histological pattern of bone marrow involvement, absolute peripheral blood lymphocytosis) or disease progression (i.e. lymphocyte doubling time), a trend toward a better life-expectancy was observed in the low CD20 expression group compared with the high CD20 expression group (p = 0.05; relative risk of death, 0.51, 95% confidence interval, 0.24-1.04). Given the correlation between CD20 ABC and mean fluorescence intensity (MFI) of light chain (LC) surface immunoglobulins (Sm Ig) (r = 0.481, p < 0.0001), as well as the impact of MFI of Sm Ig LC on overall survival (p = 0.01; relative risk of death 0.44; 95% confidence interval, 0.10 to 0.76), we tried to verify whether a combination of B-cell markers, evaluated in a quantitative manner, could have additive progn...Continue Reading

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Citations

Jun 15, 2007·Cytometry. Part B, Clinical Cytometry·Eva D RossmannAnders Osterborg
Aug 1, 2020·The Cochrane Database of Systematic Reviews·Nina KreuzbergerNicole Skoetz
Jan 23, 1999·Current Opinion in Hematology·P M LydyardP Y Youinou
Nov 23, 2005·Expert Opinion on Therapeutic Targets·Angela D Hamblin, Terry J Hamblin
May 1, 2007·Expert Review of Clinical Immunology·Amani MankaiPierre Youinou
Nov 17, 2001·American Journal of Clinical Pathology·J L FraterE D Hsi
Mar 15, 2006·Immunological Investigations·Scott H OlejniczakMyron S Czuczman

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