Clipping of Telomere from Human Chromosomes Using a Chemistry-Based Artificial Restriction DNA Cutter

Current Protocols in Nucleic Acid Chemistry
Takumi IshizukaMakoto Komiyama

Abstract

The detection of individual telomere lengths of human chromosomes can provide crucial information on genome stability, cancer, and telomere-related diseases. However, current methods to measure telomere length entail shortcomings that have limited their use. Recently, we have developed a method for detection of individual telomere lengths (DITL) that uses a chemistry-based DNA-cutting approach. The most beneficial feature of the DITL approach is to cleave the sequence adjacent to the telomere followed by resolution of the telomere length at the nucleotide level of a single chromosome. In this unit, a protocol for successful detection of individual telomere lengths from individual chromosomes is described in detail.

References

May 31, 1990·Nature·C B HarleyC W Greider
May 1, 1996·Human Molecular Genetics·P M LansdorpH J Tanke
Sep 23, 1997·BioTechniques·J E BryantJ K Griffith
Sep 27, 2001·Cell·E H Blackburn
May 10, 2002·Nucleic Acids Research·Richard M Cawthon
Jul 26, 2002·BioTechniques·Colleen A FordyceJeffrey K Griffith
Jan 23, 2003·Nature Genetics·Duncan M BairdDavid Kipling
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Titia de Lange
Sep 2, 2005·Nature Reviews. Genetics·Maria A Blasco
Feb 12, 2008·Current Protocols in Molecular Biology·T Brown
Apr 5, 2008·Nature Protocols·Makoto KomiyamaJun Sumaoka
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·Dwaine A BraaschDavid R Corey
Jun 14, 2008·Current Protocols in Nucleic Acid Chemistry·Colette M CastleberryPatrick A Limbach
Oct 30, 2009·Chemical Communications : Chem Comm·Kenichiro ItoMakoto Komiyama
May 14, 2011·Chemical Society Reviews·Yuichiro AibaMakoto Komiyama
Dec 21, 2012·Journal of the American Chemical Society·Takumi IshizukaMakoto Komiyama
Feb 7, 2013·International Journal of Molecular Sciences·Makoto Komiyama
Dec 4, 2013·Methods in Molecular Biology·Kenichiro Ito, Makoto Komiyama

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Citations

Sep 22, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Narumi ShigiMakoto Komiyama

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