Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification

Nature
Sven ReischauerD Y Stainier

Abstract

Vascular and haematopoietic cells organize into specialized tissues during early embryogenesis to supply essential nutrients to all organs and thus play critical roles in development and disease. At the top of the haemato-vascular specification cascade lies cloche, a gene that when mutated in zebrafish leads to the striking phenotype of loss of most endothelial and haematopoietic cells and a significant increase in cardiomyocyte numbers. Although this mutant has been analysed extensively to investigate mesoderm diversification and differentiation and continues to be broadly used as a unique avascular model, the isolation of the cloche gene has been challenging due to its telomeric location. Here we used a deletion allele of cloche to identify several new cloche candidate genes within this genomic region, and systematically genome-edited each candidate. Through this comprehensive interrogation, we succeeded in isolating the cloche gene and discovered that it encodes a PAS-domain-containing bHLH transcription factor, and that it is expressed in a highly specific spatiotemporal pattern starting during late gastrulation. Gain-of-function experiments show that it can potently induce endothelial gene expression. Epistasis experiments...Continue Reading

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References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C G BegleyI R Kirsch
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·J PengG H Fong
Jul 19, 2001·Annals of the New York Academy of Sciences·G LacaudG Keller
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Janet Rossant, Lorraine Howard
Aug 28, 2003·Developmental Biology·Holly A FieldDidier Y R Stainier
Dec 25, 2004·Developmental Biology·Kimberly A DooleyLeonard I Zon
Oct 28, 2005·Development·Suk-Won JinDidier Y R Stainier
Dec 13, 2005·PLoS Biology·Saulius Sumanas, Shuo Lin
Jul 11, 2006·Developmental Cell·Diana L Ramírez-BergeronM Celeste Simon
May 1, 2007·Genomics·Simona PiskacekMartin Piskacek
May 25, 2007·Nature Reviews. Molecular Cell Biology·Ralf H Adams, Kari Alitalo
Aug 8, 2007·Developmental Cell·Jeffrey J SchoenebeckDeborah Yelon
Jan 15, 2008·Nature Protocols·Christine Thisse, Bernard Thisse
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Sep 26, 2008·Nature·Yingxi LinMichael E Greenberg
Feb 17, 2009·Developmental Cell·Sarah De Val, Brian L Black
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Apr 12, 2011·Nature Methods·Guokai ChenJames A Thomson
Jun 24, 2011·Bioinformatics·Adam RobertsLior Pachter
Aug 24, 2011·Nature Reviews. Molecular Cell Biology·Shane P Herbert, Didier Y R Stainier
Sep 2, 2011·Nucleic Acids Research·Hana YiJongsik Chun
Nov 24, 2011·Genome Research·Andrea PauliAlexander F Schier
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Mar 15, 2012·Blood·Karen K Hirschi
Sep 25, 2012·Nature·Victoria M BedellStephen C Ekker
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Li-En JaoWenbiao Chen

❮ Previous
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Citations

Jan 14, 2017·Cardiovascular Research·Jennifer Susanne EsserMartin Moser
Mar 11, 2017·Developmental Biology·Satish Casie ChettySaulius Sumanas
Feb 14, 2018·Current Opinion in Hematology·Ryota L Matsuoka, Didier Y R Stainier
Feb 13, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Aniket V GoreBrant M Weinstein
May 5, 2017·Physiological Reviews·Philipp GutRima Arnaout
Jun 20, 2018·ELife·Yu Hsuan Carol YangDidier Yr Stainier
Mar 13, 2019·PloS One·Mason PosnerAndor J Kiss
Mar 3, 2019·Biomedicines·Panagiota Giardoglou, Dimitris Beis
May 22, 2019·PLoS Biology·Gaurav ByagathvalliM Saad Bhamla
Dec 20, 2019·Development·Isao KobayashiMasaaki Yamaguchi
Oct 12, 2018·Nature Communications·Oliver A StoneDidier Y R Stainier
Dec 15, 2018·Phytotherapy Research : PTR·Fatemeh SharifpanahHeinrich Sauer
Aug 29, 2019·International Journal of Molecular Sciences·Baubak BajoghliNarges Aghaallaei
Jun 25, 2020·Scientific Reports·Megan L WeaverJakub K Famulski
May 26, 2017·Nature Reviews. Molecular Cell Biology·Michael Potente, Taija Mäkinen
May 10, 2019·Frontiers in Cell and Developmental Biology·Craig M NelsonJohn R Henley
Oct 5, 2017·Nature Communications·Danhua ZhangP Duc Si Dong
Jun 21, 2020·Development·Karin D PrummelChristian Mosimann
Aug 15, 2020·Frontiers in Physiology·Kazuhide S Okuda, Benjamin M Hogan
Feb 15, 2020·Haematologica·Wei WengGuojun Sheng
Feb 16, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Saireudee ChaturantabutWolfram Goessling
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Boryeong PakSuk-Won Jin
Jan 16, 2021·Angiogenesis·Leah J Greenspan, Brant M Weinstein
Sep 25, 2020·Developmental Biology·Claudia CarlantoniDidier Y R Stainier
Jun 13, 2019·Cell Reports·Donna J PageShane P Herbert
Sep 16, 2020·Trends in Genetics : TIG·Zacharias Kontarakis, Didier Y R Stainier
Feb 13, 2021·Journal of Cardiovascular Development and Disease·Kendall E Martin, Joshua S Waxman
Nov 8, 2019·Developmental Biology·Alethia VillasenorDidier Y R Stainier

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