PMID: 6976317Dec 1, 1981Paper

Clonal analysis of B- and T-cell responses to Ia antigens. I. Topology of epitope regions on I-Ak and I-Ek molecules analyzed with 35 monoclonal alloantibodies

Immunogenetics
M PierresS Marchetto

Abstract

Thirty-five Iak-specific monoclonal alloantibodies, derived from hybridomas constructed by fusion between mouse myeloma and spleen cells from A.TH alloimmune mice (Is anti-Ik), have been used to estimate the allotypic polyporphism of the Ik-gene products. Cross-blocking studies using 17 mAb specific for the I-A molecule indicated that six determinants, which were associated with the conventional specificities Ia.2 and Ia.19, were organized in at least three distinct polymorphic areas of the I-Ak molecules. Similarly, another group of six determinants, which did not correspond to previously described conventional Ia specificities, were found to be topologically heterogeneous. By contrast, the five epitopes associated with the Ia. 1 specificity were clustered into a single region of this molecule. In addition the potentiation of binding observed between mAb specific for topologically distinct epitope regions of the I-Ak molecule, suggested that the latter may undergo conformational changes after binding of a given mAb. A similar analysis of 17 mAb specific for the I-Ek molecule indicated that specificity Ia. 7 of the E alpha chain (as defined in this series by eight mAb) was composed of three topologically distinct polymorphic ar...Continue Reading

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M PierresR N Germain
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Citations

Jan 1, 1989·Immunogenetics·V Hauptfeld-Dolejsek, D C Shreffler
Jul 1, 1987·European Journal of Immunology·I Kedar, A D Steinberg
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·A Ben Younès-ChennoufiM Hontebeyrie-Joskowicz
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Nov 10, 1983·Nature·R N GermainD H Margulies
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Jun 11, 2002·The Journal of Biological Chemistry·Carlos VelazquezEmil R Unanue
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eduardo Martinez-SoríaShozo Izui
Jun 21, 1990·Nature·R M SlatteryJ F Miller
Oct 1, 1986·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M A Norcross
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Apr 1, 1986·Immunological Reviews·J A BluestoneD H Sachs
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Apr 1, 1996·European Journal of Immunology·M Merkenschlager
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Oct 1, 1988·Journal of Immunogenetics·C DelarbreG Gachelin

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